经颅光生物调节通过改变抑郁模型小鼠大脑中的血清素和一氧化氮水平预防焦虑和抑郁：三种不同剂量810纳米激光的研究
Transcranial photobiomodulation prevents anxiety and depression via changing serotonin and nitric oxide levels in brain of depression model mice: A study of three different doses of 810 nm laser.
作者信息
Eshaghi Emad, Sadigh-Eteghad Saeed, Mohaddes Gisou, Rasta Seyed Hossein
机构信息
Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Medical Physics, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Lasers Surg Med. 2019 Sep;51(7):634-642. doi: 10.1002/lsm.23082. Epub 2019 Mar 18.
OBJECTIVES
The effectiveness of transcranial photobiomodulation (TPBM) in treating anxiety and depression disorders is a demonstrated and identified issue. However, the optimum therapeutic dose and the underlying mechanism of action are not fully understood. In this study, the therapeutic effects of three different near-infrared (NIR) doses on anxiety- and depression-like behaviors as well as cerebral levels of serotonin (5-HT) and nitric oxide (NO) were evaluated in a mouse model of chronic restraint stress (CRS).
MATERIALS AND METHODS
CRS procedure (3 hours/day, over 3 weeks) was performed as a typical stress model to study anxiety and depression along with laser treatment (3 times/week, over 3 weeks), which began simultaneously with CRS. A NIR diode laser (810 nm wavelength, 10 Hz) with the output power of 200 mW and power density of 4.75 W/cm was implemented to deliver three different doses of 4, 8, and 16 J/cm to the cerebral cortex of mice. Behavioral experiments including open field, tail suspension, and elevated plus maze tests as well as serum cortisol levels were assessed to evaluate the anti-anxiety and anti-depressive effects of NIR TPBM. The changes of 5-HT and NO levels in the prefrontal cortex (PFC) and hippocampus (Hipp) were assessed.
RESULTS
CRS procedure induced anxiety- and depression-like behaviors, increased serum cortisol levels, decreased 5-HT and increased NO levels in the PFC and Hipp areas. NIR TPBM improved behavioral results, decreased serum cortisol levels, increased 5-HT and decreased NO concentrations in the PFC and Hipp. A dose of 8 J/cm of NIR TPBM showed the maximum effects on behavioral and molecular results, while a decline was observed from the optimum effects at both lower (4 J/cm ) and higher (16 J/cm ) doses.
CONCLUSION
Our results demonstrated that NIR TPBM had an anti-anxiety and anti-depressive effect in CRS mice, which is probably linked to increasing 5-HT and decreasing NO levels in the PFC and Hipp areas. Also, the maximum anti-anxiety and anti-depressive effect was produced at dose of 8 J/cm . Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.
目的
经颅光生物调节疗法（TPBM）治疗焦虑症和抑郁症的有效性已得到证实和确认。然而，最佳治疗剂量及其潜在作用机制尚未完全明确。在本研究中，我们在慢性束缚应激（CRS）小鼠模型中评估了三种不同近红外（NIR）剂量对焦虑样和抑郁样行为以及大脑中血清素（5-HT）和一氧化氮（NO）水平的治疗效果。
材料与方法
采用CRS程序（每天3小时，持续3周）作为典型应激模型来研究焦虑和抑郁，并同时进行激光治疗（每周3次，持续3周）。使用输出功率为200 mW、功率密度为4.75 W/cm²的近红外二极管激光器（波长810 nm，频率10 Hz），向小鼠大脑皮层输送三种不同剂量（4、8和16 J/cm²）的能量。通过旷场试验、悬尾试验和高架十字迷宫试验等行为学实验以及血清皮质醇水平评估，来评价近红外TPBM的抗焦虑和抗抑郁作用。同时评估前额叶皮质（PFC）和海马体（Hipp）中5-HT和NO水平的变化。
结果
CRS程序诱导了焦虑样和抑郁样行为，增加了血清皮质醇水平，降低了PFC和Hipp区域的5-HT水平，并升高了NO水平。近红外TPBM改善了行为学结果，降低了血清皮质醇水平，增加了PFC和Hipp区域的5-HT水平，并降低了NO浓度。8 J/cm²的近红外TPBM剂量对行为学和分子学结果显示出最大影响，而在较低（4 J/cm²）和较高（16 J/cm²）剂量下，效果均从最佳效果有所下降。
结论
我们的结果表明，近红外TPBM对CRS小鼠具有抗焦虑和抗抑郁作用，这可能与增加PFC和Hipp区域的5-HT水平以及降低NO水平有关。此外，8 J/cm²的剂量产生了最大的抗焦虑和抗抑郁效果。《激光外科与医学》。© 2019威利期刊公司。
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