Salehpour Farzad, Rasta Seyed Hossein, Mohaddes Gisou, Sadigh-Eteghad Saeed, Salarirad Sima
Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz 51666, Iran.
Department of Medical Physics, Tabriz University of Medical Sciences, Tabriz 51666, Iran.
Lasers Surg Med. 2016 Sep;48(7):695-705. doi: 10.1002/lsm.22542. Epub 2016 Jul 1.
The application of transcranial low-level light/laser therapy (tLLLT) in the range of red to near-infrared (NIR) spectrum for psychological disorders is a new area that is attracting growing interest in recent years. The photomodulation effects of NIR and red coherent lights on the activity of cytochrome c oxidase in neuronal cells of brain have been recently introduced. This study, therefore, sought to compare the therapeutic effects of 10-Hz pulsed wave NIR (810 nm) laser with red (630 nm) laser using the same delivered energy density and Citalopram in rat chronic mild stress (CMS) model of depression and anxiety.
CMS procedures (for 4 weeks) were used to induce stress. GaAlAs diode laser with red and NIR wavelengths on 10-Hz pulsed wave (50% duty cycle) were used to perform tLLLT treatment for three weeks. An energy density of about 1.2 J/cm-(2) per each session was delivered through a light spot with a diameter of 3-mm to the prefrontal cortex for both wavelengths. Citalopram (10 mg/kg, Intraperitoneal) was administered for twenty-one consecutive days to the drug group.
The findings of the present study showed an increase in swimming and decrease in immobility time, for both NIR laser and Citalopram groups compared to the stress group in forced swimming test. Anxiety-like behaviors showed insignificant decrease in all treatment groups in elevated plus maze test. The induction of stress significantly increased serum cortisol levels and treatments with both red laser and Citalopram decreased it. Hyperglycemia induced by CMS returned to normal levels in all treatment groups. The assessment of body weight also showed a significant increase in NIR laser group compared to the stress group by the end of the experiment.
This study showed that non-invasive tLLLT using 10-Hz pulsed NIR laser light was as effective as Citalopram and more effective than red laser in the treatment of depressive-like behaviors and may help improve tLLLT as an alternative non-pharmacological treatments of psychological disorders such as depression. Lasers Surg. Med. 48:695-705, 2016. © 2016 Wiley Periodicals, Inc.
经颅低强度光/激光疗法（tLLLT）在红色至近红外（NIR）光谱范围内用于治疗心理障碍是一个近年来日益受到关注的新领域。近年来已发现近红外和红色相干光对大脑神经元细胞中细胞色素c氧化酶活性具有光调制作用。因此，本研究旨在比较在相同的能量密度下，10赫兹脉冲波近红外（810纳米）激光与红色（630纳米）激光以及西酞普兰对大鼠慢性轻度应激（CMS）抑郁和焦虑模型的治疗效果。
采用CMS程序（持续4周）诱导应激。使用波长为红色和近红外的10赫兹脉冲波（占空比50%）的GaAlAs二极管激光进行为期三周的tLLLT治疗。每次治疗通过直径为3毫米的光斑将约1.2焦耳/平方厘米的能量密度传递至前额叶皮层，两种波长均如此。药物组连续21天腹腔注射西酞普兰（10毫克/千克）。
本研究结果显示，在强迫游泳试验中，与应激组相比，近红外激光组和西酞普兰组的游泳时间增加，不动时间减少。在高架十字迷宫试验中，所有治疗组的焦虑样行为均有不显著的减少。应激诱导显著提高了血清皮质醇水平，红色激光和西酞普兰治疗均使其降低。CMS诱导的高血糖在所有治疗组中均恢复至正常水平。体重评估还显示，到实验结束时，近红外激光组与应激组相比体重显著增加。
本研究表明，使用10赫兹脉冲近红外激光进行的非侵入性tLLLT在治疗抑郁样行为方面与西酞普兰效果相当，且比红色激光更有效，可能有助于改进tLLLT作为抑郁症等心理障碍的替代非药物治疗方法。《激光外科与医学》第48卷：695 - 705页（2016年）。©2016威利期刊公司
