Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260 Concepción del Uruguay, Entre Ríos, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Laboratorio de Sanidad Aviar, Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260 Concepción del Uruguay, Entre Ríos, Argentina.
Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260 Concepción del Uruguay, Entre Ríos, Argentina.
Food Res Int. 2019 May;119:541-546. doi: 10.1016/j.foodres.2017.08.008. Epub 2017 Aug 4.
This study was conducted to estimate the apparent prevalence of Salmonella spp. in chicken livers obtained from markets in Entre Ríos, Argentina, using two culture methods (preenrichment and direct selective agar plating). We also determined the antimicrobial resistance of Salmonella isolated strains and evaluated the performance of the two culture methods and selective-differential plating media used for Salmonella isolation. Of 666 chicken livers studied, 32 organs (4.8%) related to 4 poultry slaughterhouse companies were positive for Salmonella sp. using one or two culture methods. Fifty Salmonella strains were isolated from the positive liver samples and were typed into 3 serovars: S. ser. Schwarzengrund (78%), S. ser. Enteritidis (18%), and S. ser. Typhimurium 4(%). More than one Salmonella serovar was found in livers belonging to two chicken slaughterhouse companies. All strains were susceptible to all antibiotics tested, with the exception of erythromycin (100% resistant) and streptomycin (22% intermediate sensitivity). Overall, 32 (4.80%) and 3 (0.45%) of the chicken liver samples were positive for Salmonella sp. in preenrichment method and direct selective agar plating method, respectively; these percentages were significantly different (P=0.0001; kappa=0.16). There was also a statistical difference in relative accuracy, sensitivity and negative predictive value between the preenrichment method and the direct selective agar plating method; the first had greater values for these parameters than the direct selective agar plating method. These parameters were statistically different between MacConkey agar (MCA) and modified lysine iron (MLIA) in the two culture methods; the second had greater values than MCA for both culture methods. This study shows that even though serovars that are important for public health were isolated, the prevalence of Salmonella sp. is low in chicken livers from Entre Rios, Argentina. The isolated strains do not have multi-resistance patterns. Furthermore, the preenrichment method and MLIA are superior to the direct selective agar plating method and MCA for Salmonella sp. isolation from chicken liver samples, respectively.
本研究旨在使用两种培养方法（预增菌和直接选择性琼脂平板）估计从阿根廷恩特雷里奥斯省市场获得的鸡肝中沙门氏菌属的明显流行率。我们还确定了分离的沙门氏菌菌株的抗菌耐药性，并评估了两种培养方法和用于沙门氏菌分离的选择性差异平板培养基的性能。在研究的 666 个鸡肝中，使用一种或两种培养方法，来自 4 个家禽屠宰场公司的 32 个器官（4.8%）与沙门氏菌属呈阳性。从阳性肝样本中分离出 50 株沙门氏菌菌株，并分为 3 个血清型：S. ser. Schwarzengrund（78%）、S. ser. Enteritidis（18%）和 S. ser. Typhimurium 4（%）。属于两个鸡屠宰场公司的肝脏中发现了不止一种沙门氏菌血清型。所有菌株对所有测试的抗生素均敏感，除了红霉素（100%耐药）和链霉素（22%中度敏感）。总体而言，预增菌法和直接选择性琼脂平板法检测到鸡肝中沙门氏菌属阳性的样本分别为 32 份（4.80%）和 3 份（0.45%）；这两个百分比有显著差异（P=0.0001；kappa=0.16）。预增菌法和直接选择性琼脂平板法之间的相对准确性、敏感性和阴性预测值也存在统计学差异；前者的这些参数值大于后者。两种培养方法中，MacConkey 琼脂（MCA）和改良赖氨酸铁琼脂（MLIA）之间的参数也存在统计学差异；后者在两种培养方法中均大于 MCA。本研究表明，尽管分离出了对公共卫生很重要的血清型，但阿根廷恩特雷里奥斯省鸡肝中沙门氏菌属的流行率较低。分离株没有多耐药模式。此外，预增菌法和 MLIA 分别优于直接选择性琼脂平板法和 MCA 用于从鸡肝样本中分离沙门氏菌属。
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