Carramiñana Juan J, Rota Carmina, Agustín I, Herrera Antonio
Department of Animal Production and Food Science, Veterinary Faculty, Food Hygiene, Inspection, Control, and Microbiology Unit, University of Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
Vet Microbiol. 2004 Nov 30;104(1-2):133-9. doi: 10.1016/j.vetmic.2004.08.010.
Salmonellosis is a major foodborne infection in Spain, and strains that are resistant to a great variety of antibiotics have become a major public health concern. The aim of this study was to determine the level of antibiotic resistance in 133 Salmonella isolates obtained from a poultry slaughterhouse in Zaragoza (NE Spain). Antimicrobial resistance testing was performed by disk diffusion method using 19 antibiotics. Results were interpreted following the NCCLS criteria. Overall, the highest percentage of resistance was found to the following antimicrobial agents: sulfadiazine (96.2%), neomycin (53.4%), tetracycline (21.8%), and streptomycin (11.3%). All isolates were found to be resistant to one or more of the antibiotics tested. Multiple resistance was observed in 87 strains (65.4%). We found 23 different patterns of resistance in Salmonella Enteritidis. Resistance to sulfadiazine was the most common single resistance. The most frequent patterns of multiresistant strains were neomycin+sulfadiazine and neomycin+tetracycline+sulfadiazine. S. 4,5,12:b: showed the highest percentages of resistance to the tested drugs, with five different resistance patterns found. Ampicillin+chloramphenicol+streptomycin+sulphonamides+tetracycline (ACSSuT) resistance pattern, commonly associated with S. Typhimurium DT 104, was not detected in strains of the same phage type from broilers. The appearance of substantial multiresistance in foodborne Salmonella isolates suggests the need for more prudent use of antibiotics by farmers, veterinarians, and physicians.
沙门氏菌病是西班牙一种主要的食源性感染疾病，对多种抗生素具有耐药性的菌株已成为一个重大的公共卫生问题。本研究的目的是确定从萨拉戈萨（西班牙东北部）一家家禽屠宰场分离出的133株沙门氏菌的抗生素耐药水平。采用纸片扩散法，使用19种抗生素进行药敏试验。结果按照美国国家临床实验室标准化委员会（NCCLS）标准进行判读。总体而言，对以下抗菌药物耐药率最高：磺胺嘧啶（96.2%）、新霉素（53.4%）、四环素（21.8%）和链霉素（11.3%）。所有分离株均对一种或多种测试抗生素耐药。87株（65.4%）观察到多重耐药。我们在肠炎沙门氏菌中发现了23种不同的耐药模式。对磺胺嘧啶的耐药是最常见的单一耐药。多重耐药菌株最常见的模式是新霉素+磺胺嘧啶以及新霉素+四环素+磺胺嘧啶。鼠伤寒沙门氏菌4,5,12:b:对测试药物的耐药率最高，发现了五种不同的耐药模式。在肉鸡相同噬菌体类型的菌株中未检测到通常与鼠伤寒沙门氏菌DT104相关的氨苄西林+氯霉素+链霉素+磺胺类药物+四环素（ACSSuT）耐药模式。食源性沙门氏菌分离株中出现大量多重耐药表明农民、兽医和医生需要更谨慎地使用抗生素。
