Anderson T D, Cheville N F
Am J Pathol. 1986 Aug;124(2):226-37.
Trophoblasts in normal and Brucella abortus-infected caprine placentas were examined by ultrastructural morphometric analysis to establish structural relationships of B abortus with cytoplasmic organelles; brucellae were identified with colloidal gold-labeled anti-B abortus bovine IgG. Cytotrophoblasts had large numbers of B abortus in cisternae of rough endoplasmic reticulum; binucleate trophoblasts did not contain bacteria. In infected trophoblasts there was a significant hypertrophy of B abortus-filled rough endoplasmic reticulum (RER) and a corresponding reduction in normal-appearing RER. Volume and surface density of RER in trophoblasts were: normal placentas (control), 2.8% and 0.30 sq mu; infected placentas, 27.9% (27.4% of which contained B abortus) and 0.56 sq mu (cells containing B abortus) and 3.3% and 0.34 sq mu (cells not containing B abortus). These data suggest that B abortus replicates within the RER of trophoblasts, possibly for synthesis and glycosylation of bacterial membrane proteins.
通过超微结构形态计量分析检查正常和感染布鲁氏菌流产亚种的山羊胎盘滋养层细胞，以确定流产布鲁氏菌与细胞质细胞器的结构关系；用胶体金标记的抗流产布鲁氏菌牛IgG鉴定布鲁氏菌。细胞滋养层细胞的粗面内质网池中有大量流产布鲁氏菌；双核滋养层细胞不含细菌。在受感染滋养层细胞中，充满流产布鲁氏菌的粗面内质网（RER）显著肥大，而外观正常的RER相应减少。滋养层细胞中RER的体积和表面密度分别为：正常胎盘（对照），2.8%和0.30平方微米；感染胎盘，27.9%（其中27.4%含有流产布鲁氏菌）和0.56平方微米（含流产布鲁氏菌的细胞）以及3.3%和0.34平方微米（不含流产布鲁氏菌的细胞）。这些数据表明，流产布鲁氏菌在滋养层细胞的RER内复制，可能用于细菌膜蛋白的合成和糖基化。
