Ziegert E, Stelzer W
Zentralbl Mikrobiol. 1986;141(2):121-8.
In this report we examined quantitatively (MPN-procedure) various methods of isolating Ps. aeruginosa from surface waters. When comparing different media (malachitgreen broth, Preuss-broth, selenite broth, asparagine broth, malachitgreen/acetamide broth) the enrichments in malachitgreen and asparagine showed the highest isolation frequencies after an 48 h incubation time. In heavily polluted river water the results can be improved by a second enrichment step in acetamide broth. Dependent on the river water pollution 0...11,000 Ps. aeruginosa/l were detected. In waste water up to 2.4 X 10(6) Ps. aeruginosa/l were isolated. The results emphasize the significance of water as a reservoir and a source of distribution of Ps. aeruginosa in the environment.
在本报告中，我们采用定量方法（MPN 法）研究了从地表水中分离铜绿假单胞菌的各种方法。比较不同培养基（孔雀绿肉汤、普雷斯肉汤、亚硒酸盐肉汤、天冬酰胺肉汤、孔雀绿/乙酰胺肉汤）时，孔雀绿肉汤和天冬酰胺肉汤在培养 48 小时后的富集培养物显示出最高的分离频率。在污染严重的河水中，通过在乙酰胺肉汤中进行第二步富集培养可提高检测结果。根据河水污染程度，检测到每升水中有 0……11,000 个铜绿假单胞菌。在废水中，每升分离出高达 2.4×10⁶个铜绿假单胞菌。结果强调了水作为铜绿假单胞菌在环境中的储存库和传播源的重要性。
