State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing Road, Haidian District, Beijing 100085, People's Republic of China.
University of Chinese Academy of Sciences, 19A Yuquan Rd, Beijing 100049, People's Republic of China.
FEMS Microbiol Ecol. 2019 Apr 1;95(4). doi: 10.1093/femsec/fiz036.
Rice plants are the habitat for large and diverse populations of microbes, which play important roles on rice health and productivity. However, the response of microbiome on rice culm to water flooding is poorly understood. In this study, the bacterial community on non-flooded (RSA) and flooded (RSB) rice culms was investigated through 16S rRNA gene sequencing. The results showed that RSA and RSB had significantly distinct bacterial communities. In RSA, Gammaproteobacteria and Pantoea were the most abundant class (57%), genus (37.06%), respectively, while in RSB, the most abundant phylum and genus was Firmicutes (54%) and Bacillus (52.63%), respectively. Compared with RSA, the abundance of 27 genera significantly increased and 21 genera significantly decreased in RSB, and some remarkably changed species, such as Aeromonas, Bacillus were identified, which are sensitive to non-flooded or flooded conditions. In addition, rare operational taxonomic units (OTUs) was much more than abundant OTUs in all samples, and RSB had significantly higher bacterial richness than RSA due to having more rare taxa. Our study would advance the insights into the microbiome of rice culms and its response to flooding, which would help to identify potential beneficial bacteria for improving crop health and sustainable productivity in agroecosystems.
水稻植株是大量多样微生物的栖息地，这些微生物对水稻的健康和生产力起着重要作用。然而，水稻茎对水淹的微生物组响应仍知之甚少。在这项研究中，通过 16S rRNA 基因测序研究了未受水淹（RSA）和受水淹（RSB）的水稻茎上的细菌群落。结果表明，RSA 和 RSB 具有明显不同的细菌群落。在 RSA 中，γ变形菌门和泛菌属分别是最丰富的类（57%）和属（37.06%），而在 RSB 中，最丰富的门和属是厚壁菌门（54%）和芽孢杆菌属（52.63%）。与 RSA 相比，RSB 中 27 个属的丰度显著增加，21 个属的丰度显著减少，鉴定出一些明显变化的物种，如气单胞菌、芽孢杆菌，它们对未受水淹或受水淹的条件敏感。此外，所有样本中的稀有分类单元（OTU）比丰富的 OTU 多得多，由于 RSB 具有更多的稀有分类群，因此其细菌丰富度显著高于 RSA。本研究将深入了解水稻茎的微生物组及其对水淹的响应，这将有助于鉴定潜在的有益细菌，以提高农业生态系统中作物的健康和可持续生产力。
