Muğla Sıtkı Koçman University, Faculty of Science, Chemistry Department, Muğla, Turkey.
Muğla Sıtkı Koçman University, Muğla Vocational School, Chemistry and Chemical Processing Technology Department, Muğla, Turkey.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:1226-1235. doi: 10.1016/j.msec.2019.02.031. Epub 2019 Feb 11.
Polyvinyl alcohol (PVA)/Zn electrospun nanofibers that were a kind of polymer/ionic metal composite was successfully embedded in the hybrid fibers for the first time in the literature, due to chemical interactions between PVA and Zn. Also, the nanofibers were used as carriers for the first time in enzyme immobilization. The nanofibers were optimized and synthesized by electrospinning technique according to the operational parameters like as PVA concentration (%), Zn concentration (%), voltage (kV), needle tip-collector distance (cm) and injection speed (ml/h). The morphology and structure of the nanofibers were characterized by SEM, XRD, ATR-FTIR and TGA. Lipase was immobilized on the nanofibers by adsorption and crosslinking methods. According to immobilization results, nanofiber enhanced enzyme stability properties like as thermal stability, pH stability and reusability. Lipase immobilized nanofiber protected 90% of its activity after 14 reuses.
聚乙烯醇（PVA）/Zn 电纺纳米纤维是一种聚合物/离子金属复合材料，由于 PVA 和 Zn 之间的化学相互作用，首次成功嵌入杂化纤维中。此外，纳米纤维首次被用作酶固定化的载体。纳米纤维通过静电纺丝技术进行优化和合成，根据操作参数如 PVA 浓度（%）、Zn 浓度（%）、电压（kV）、针尖-收集器距离（cm）和注射速度（ml/h）。纳米纤维的形态和结构通过 SEM、XRD、ATR-FTIR 和 TGA 进行表征。脂肪酶通过吸附和交联法固定在纳米纤维上。根据固定化结果，纳米纤维增强了酶的稳定性，如热稳定性、pH 稳定性和可重复使用性。固定化脂肪酶的纳米纤维在 14 次重复使用后仍保持 90%的活性。
