Faculty of Science, Chemistry Department, Muğla Sıtkı Koçman University, Muğla, Turkey.
Chemistry and Chemical Processing Technology Department, Muğla Vocational School, Muğla Sıtkı Koçman University, Muğla, Turkey.
Prep Biochem Biotechnol. 2020;50(5):425-437. doi: 10.1080/10826068.2019.1679175. Epub 2020 Apr 1.
Electrospun polyvinyl alcohol (PVA)/Chitosan nanofibers were successfully prepared and were used as carriers for the first time in urease immobilization. Also, urease immobilized electrospun PVA/Chitosan nanofibers were applied for the removal of urea from artificial blood serum by recycled reactor. The nanofibers were optimized and synthesized by electrospinning technique according to the operational parameters. The morphology and structure of the nanofibers were characterized by scanning electron microscopy (SEM), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and thermogravimetric analysis (TGA). Urease was immobilized on the nanofibers by adsorption and crosslinking methods. According to immobilization results, nanofiber enhanced urease stability properties like thermal stability, pH stability, and reusability. Urease immobilized electrospun PVA/Chitosan nanofiber protected its activity by 85% after 10 uses and 45% after 20 uses. Urea removal rates of artificial blood serum were as follows: 100% at 1st cycle, 95% at 2nd, 3rd and 4th cycles; 85% at the 5th cycle; 76% at the 6th cycle, and 65% at the last three cycles.
静电纺丝聚乙烯醇（PVA）/壳聚糖纳米纤维的成功制备，并首次将其用作固定化脲酶的载体。此外，固定化脲酶的静电纺丝 PVA/壳聚糖纳米纤维被应用于通过循环反应器从人工血清中去除尿素。纳米纤维通过静电纺丝技术根据操作参数进行优化和合成。通过扫描电子显微镜（SEM）、衰减全反射傅里叶变换红外光谱（ATR-FTIR）和热重分析（TGA）对纳米纤维的形态和结构进行了表征。通过吸附和交联方法将脲酶固定在纳米纤维上。根据固定化结果，纳米纤维增强了脲酶的稳定性，如热稳定性、pH 稳定性和可重复使用性。固定化脲酶的静电纺丝 PVA/壳聚糖纳米纤维在第 10 次和第 20 次使用后，其活性分别保护了 85%和 45%。人工血清中尿素的去除率如下：第 1 次循环为 100%，第 2、3 和 4 次循环为 95%；第 5 次循环为 85%；第 6 次循环为 76%，最后三次循环为 65%。
