Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin 541004, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 May 5;272:121008. doi: 10.1016/j.saa.2022.121008. Epub 2022 Feb 11.
Nanocatalytic amplification of carbon dots is a new way to improve sensitivity. The preparation of the high catalytic activity and stable iron/nitrogen-doped carbon dot (CD) sol and its application in aptamer (Apt) assay have not been reported yet. In this paper, a simple hydrothermal procedure for the preparation of CD derived from Fe-2,2'-bipyridine complex has been developed. It is found that CD has a strong catalytic effect on the indicator reaction of glyoxal (CHO) reduction of HAuCl to produce gold nanoparticle (AuNP) probe with strong surface enhanced Raman scattering (SERS) at 1617 cm, resonance Rayleigh scattering (RRS) effect at 370 nm and absorption (Abs) at 550 nm. A rapid and sensitive CD catalytic amplification Apt method for SERS/RRS/Abs trimode detecting ultratrace lead ions was established, based on the Apt reaction mediated the nanocatalytic indicator reaction. The results show that the SERS intensity and Pb(II) concentration have a good linear relationship in the range of 1.3-16 pM, and the detection limit is the lowest. In addition, Hg(II) and As(III) can also be measured by this naocatalytic amplification- Apt assay platform.
纳米催化放大碳点是提高灵敏度的一种新方法。高催化活性和稳定的铁/氮掺杂碳点（CD）溶胶的制备及其在适体（Apt）分析中的应用尚未见报道。本文开发了一种简单的水热法从 Fe-2,2'-联吡啶配合物制备 CD。研究发现，CD 对乙二醛（CHO）还原 HAuCl 生成金纳米粒子（AuNP）探针的指示剂反应具有很强的催化作用，在 1617 cm 处具有很强的表面增强拉曼散射（SERS）、370 nm 处的共振瑞利散射（RRS）效应和 550 nm 处的吸收（Abs）。基于适体反应介导的纳米催化指示剂反应，建立了一种快速灵敏的 CD 催化放大适体用于 SERS/RRS/Abs 三模式检测痕量铅离子的方法。结果表明，在 1.3-16 pM 范围内，SERS 强度与 Pb(II)浓度具有良好的线性关系，检测限最低。此外，该纳米催化放大-适体分析平台还可以测定 Hg(II)和 As(III)。
