Department of Ophthalmology, 5323 Harry Hines Blvd, Dallas, TX, 75390-9057, USA; Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX, 75390-9057, USA.
Department of Ophthalmology, 5323 Harry Hines Blvd, Dallas, TX, 75390-9057, USA.
Ocul Surf. 2019 Oct;17(4):793-808. doi: 10.1016/j.jtos.2019.03.002. Epub 2019 Mar 16.
The possible role of sex in the biosynthesis of lipids in the Meibomian glands (termed meibogenesis) remains unclear. To determine if there were any major sex-specific differences in the lipid composition of meibomian gland secretions (meibum) and gene expression patterns (GEP) related to meibogenesis, we conducted a study using healthy, age and diet-matched young adult wild-type C57BL/6J mice (2-2.5 month old). Tarsal plates (TP) were surgically excised from the eyelids of mice and subjected to transcriptomic and lipidomic analyses. The GEP were studied using mRNA microarrays. Lipids were extracted with organic solvents and analyzed using liquid chromatography and mass spectrometry. GEP in the TP of female and male mice demonstrated no statistically significant differences in the expression levels of the main protein-coding genes related to lipid metabolism and storage in general, and meibogenesis specifically (such as Elovl, Scd, Fads, Soat, Far, Awat, Acat, Lss, Dhcr, Hmgcr, Hmgcs, Dgat, Bckdh, Dbt, Fasn, and Plin, among others). The meibomian lipid profiles of female and male mice were virtually indistinguishable: all major lipids such as waxes, cholesteryl esters, cholesterol, (O)-acylated omega-hydroxy fatty acids (OAHFA), cholesteryl esters of OAHFA etc., were present in similar ratios. It seems that the major biosynthetic pathways in the Meibomian glands of male and female mice function in a similar fashion and produce secretions of the same overall chemical composition.
性在睑板腺（称为睑板生成）的脂质生物合成中可能发挥的作用尚不清楚。为了确定睑板腺分泌物（睑脂）的脂质组成和与睑板生成相关的基因表达模式（GEP）是否存在任何主要的性别特异性差异，我们使用健康、年龄和饮食匹配的年轻成年野生型 C57BL/6J 小鼠（2-2.5 月龄）进行了一项研究。通过手术从小鼠的眼睑中切除睑板，并对其进行转录组学和脂质组学分析。GEP 使用 mRNA 微阵列进行研究。用有机溶剂提取脂质，并使用液相色谱和质谱进行分析。雌性和雄性小鼠的 TP 中的 GEP 在与脂质代谢和储存相关的主要蛋白质编码基因的表达水平上没有表现出统计学上的显著差异，特别是在睑板生成方面（如 Elovl、Scd、Fads、Soat、Far、Awat、Acat、Lss、Dhcr、Hmgcr、Hmgcs、Dgat、Bckdh、Dbt、Fasn 和 Plin 等）。雌性和雄性小鼠的睑脂谱几乎无法区分：所有主要的脂质，如蜡、胆固醇酯、胆固醇、（O）酰化 ω-羟基脂肪酸（OAHFA）、OAHFA 的胆固醇酯等，都以相似的比例存在。似乎男性和女性小鼠的睑板腺中的主要生物合成途径以相似的方式发挥作用，并产生相同总体化学成分的分泌物。
