Arita Reiko, Mori Naoto, Shirakawa Rika, Asai Kei, Imanaka Takahiro, Fukano Yasufumi, Nakamura Masatsugu
Department of Ophthalmology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan; Department of Ophthalmology, Itoh Clinic, 626-11 Minaminakano, Minuma-ku, Saitama-shi, Saitama, 337-0042, Japan.
Research and Development Division, Santen Pharmaceutical Co. Ltd., 4-20 Ofuka-cho, Kita-ku, Osaka, 530-8552, Japan.
Exp Eye Res. 2016 Apr;145:359-362. doi: 10.1016/j.exer.2016.02.005. Epub 2016 Feb 24.
To examine the relation between changes in the free fatty acid (FFA) composition of human meibum and both objective signs and subjective symptoms of meibomian gland dysfunction (MGD), we analyzed the FFA content of meibum collected from both MGD patients and control subjects. Thirty-eight patients with MGD (13 men and 25 women; mean age ± SD, 66.9 ± 15.0 years) were evaluated. Various objective signs and subjective symptoms of MGD were assessed. Meibum was analyzed by liquid chromatography-Fourier transform mass spectrometry, and the relation between the FFA composition of meibum and each objective sign and subjective symptom was examined by principal component analysis (PCA). No relation was apparent between the FFA composition of meibum and individual subjective symptoms or objective signs of MGD. However, a PCA score plot for meibum samples grouped on the basis of the severity of both telangiectasia and plugging of meibomian gland orifices revealed clear separation of mild and severe groups. This separation of the two groups was largely due to a significantly increased linoleic acid content in meibum of the severe group (3.56%, versus 0.70% of total FFAs in the mild group). The relative amount of linoleic acid in meibum was thus associated with the severity of telangiectasia and plugging of gland orifices in MGD, suggesting that this FFA might contribute to the pathogenesis of these signs.
为了研究人睑脂中游离脂肪酸（FFA）组成的变化与睑板腺功能障碍（MGD）的客观体征和主观症状之间的关系，我们分析了MGD患者和对照受试者睑脂中的FFA含量。对38例MGD患者（13例男性和25例女性；平均年龄±标准差，66.9±15.0岁）进行了评估。评估了MGD的各种客观体征和主观症状。通过液相色谱-傅里叶变换质谱法分析睑脂，并通过主成分分析（PCA）研究睑脂中FFA组成与每种客观体征和主观症状之间的关系。睑脂中FFA组成与MGD的个体主观症状或客观体征之间未发现明显关系。然而，根据睑板腺毛细血管扩张和开口堵塞的严重程度对睑脂样本进行分组的PCA得分图显示，轻度和重度组有明显分离。两组的这种分离主要是由于重度组睑脂中亚油酸含量显著增加（3.56%，而轻度组总FFA中亚油酸含量为0.70%）。因此，睑脂中亚油酸的相对含量与MGD中毛细血管扩张和腺体开口堵塞的严重程度相关，表明这种FFA可能有助于这些体征的发病机制。
