Zhang Yanni, Li Zhongxin, Zhao Zhaolong, Kuai Wentao, Wei Cong, Lv Jian, Zhi Jie, Jia Yitao
Second Department of Surgery, the Forth Hospital of Hebei Medical University, 169 Tianshan Road, Shijiazhuang, Hebei 050000, China.
Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
Evid Based Complement Alternat Med. 2019 Feb 12;2019:3870812. doi: 10.1155/2019/3870812. eCollection 2019.
Fatigue is a common, distressing, and persistent symptom for patients with malignant tumor including colorectal cancer (CRC). Although studies of cancer-related fatigue (CRF) have sprung out in recent years, the pathophysiological mechanisms that induce CRF remain unclear, and effective therapeutic interventions have yet to be established.
To investigate the effect of the traditional Chinese medicine YangZheng XiaoJi (YZXJ) on CRF, we constructed orthotopic colon cancer mice, randomly divided into YZXJ group and control (NS) group. Physical or mental fatigue was respectively assessed by swimming exhaustion time or suspension tail resting time. At the end of the experiment, serum was collected to measure the expression level of inflammatory factors by ELISA and feces to microbiota changes by 16s rDNA, and hepatic glycogen content was detected via the anthrone method.
The nutritional status of the YZXJ group was better than that of the control group, and there was no statistical difference in tumor weight. The swimming exhaustion times of YZXJ group and control group were (162.80 ± 14.67) s and (117.60 ± 13.42, < 0.05) s, respectively; the suspension tail resting time of YZXJ group was shorter than that of the control group (49.85 ± 4.56) s and (68.83 ± 7.26) s, < 0.05)). Serum levels of IL-1 and IL-6 in YZXJ group were significantly lower than the control group ( < 0.05). Liver glycogen in YZXJ group was (5.18 ± 3.11) mg/g liver tissue, which was significantly higher than that in control group (2.95 ± 2.06) mg/g liver tissue ( < 0.05). At phylum level, increased abundance of and and decreased in YZXJ group emerged as the top differences between the two groups, and the ratio was decreased in YZXJ group compared to the control group. At genus level, the abundance of , unidentified and which all belong to phylum were increased, while , and in phylum were decreased after YZXJ intervention. YZXJ can also increase , and and decrease and .
YZXJ may reduce the physical and mental fatigue caused by colorectal cancer by inhibiting inflammatory reaction, promoting hepatic glycogen synthesis, and changing the composition of intestinal microbiota.
疲劳是包括结直肠癌（CRC）在内的恶性肿瘤患者常见、令人痛苦且持续存在的症状。尽管近年来关于癌症相关疲劳（CRF）的研究大量涌现，但诱发CRF的病理生理机制仍不清楚，有效的治疗干预措施也尚未确立。
为研究中药扶正消积（YZXJ）对CRF的影响，我们构建了原位结肠癌小鼠模型，随机分为YZXJ组和对照组（生理盐水组）。分别通过游泳耗竭时间或悬尾静止时间评估身体或精神疲劳程度。实验结束时，收集血清用酶联免疫吸附测定法（ELISA）检测炎症因子表达水平，收集粪便用16s核糖体DNA检测微生物群变化，并用蒽酮法检测肝糖原含量。
YZXJ组的营养状况优于对照组，肿瘤重量无统计学差异。YZXJ组和对照组的游泳耗竭时间分别为（162.80±14.67）秒和（117.60±13.42，<0.05）秒；YZXJ组的悬尾静止时间短于对照组（49.85±4.56）秒和（68.83±7.26）秒，<0.05）。YZXJ组血清白细胞介素-1（IL-1）和白细胞介素-6（IL-6）水平显著低于对照组（<0.05）。YZXJ组肝糖原含量为（5.18±3.11）毫克/克肝组织，显著高于对照组（2.95±2.06）毫克/克肝组织（<0.05）。在门水平上，YZXJ组中厚壁菌门和放线菌门丰度增加、拟杆菌门减少是两组之间最显著的差异，且YZXJ组中厚壁菌门与拟杆菌门的比例低于对照组。在属水平上，YZXJ干预后，所有属于厚壁菌门的粪杆菌属、未鉴定的瘤胃球菌属和双歧杆菌属丰度增加，而拟杆菌门中的拟杆菌属、普雷沃菌属和嗜胆菌属丰度降低。YZXJ还可增加阿克曼菌属、理研菌科和双歧杆菌属丰度并降低脱硫弧菌属和大肠杆菌属丰度。
YZXJ可能通过抑制炎症反应、促进肝糖原合成以及改变肠道微生物群组成来减轻结直肠癌引起的身体和精神疲劳。
