Dogan Halef, Timurkaan Necati, Saat Nevzat, Seker Ibrahim, Risvanli Ali
Department of Obstetrics and Gynecology Faculty of Veterinary Medicine University of Firat Elazig Turkey.
Department of Pathology Faculty of Veterinary Medicine University of Firat Elazig Turkey.
Animal Model Exp Med. 2018 Sep 25;1(3):242-245. doi: 10.1002/ame2.12027. eCollection 2018 Sep.
The aim of the present study was to develop a new animal model for use in uterine torsion, uterine ischemia-reperfusion, and fetal hypoxia studies in rats. A total of 14 pregnant rats on their 18th-19th gestational days were used. The animals were randomly divided into two groups: those undergoing the shame operation (group 1), and those in which a 360 uterine torsion was performed using a novel technique, which was corrected 6 hours later (group 2). Subsequently, seven female and seven male rat pups aged 1 month were separated from the mothers in each group. The female rats were monitored until puberty via measuring the vaginal apertures. The 1-month old male rats and the female rats on reaching puberty were decapitated and histopathological tests were performed on the dissected organs, including the cerebral, visceral and genital organs. At the end of the study, no differences were observed between the groups with regard to abortions, offspring death rates and congenital abnormalities. It was observed that the time to reach puberty in female rats born from mothers with uterine torsion was longer, but the difference was statistically insignificant. No microscopic lesions were detected in the cerebral, visceral or genital organs of the offspring. Accordingly, it was concluded that offspring of mothers with the uterine torsion were not affected, at least in the short term. It was generally concluded that this animal model is appropriate for use in uterine torsion and ischemia-reperfusion studies, but is not appropriate for fetal hypoxia studies.
本研究的目的是开发一种新的动物模型，用于大鼠子宫扭转、子宫缺血再灌注和胎儿缺氧研究。总共使用了14只处于妊娠第18 - 19天的怀孕大鼠。这些动物被随机分为两组：进行假手术的组（第1组），以及使用新技术进行360度子宫扭转并在6小时后纠正的组（第2组）。随后，从每组的母鼠中分离出7只1月龄的雌性和7只雄性幼鼠。通过测量阴道口对雌性大鼠进行监测直至青春期。对1月龄雄性大鼠和达到青春期的雌性大鼠进行断头处理，并对解剖的器官进行组织病理学检查，包括脑、内脏和生殖器官。在研究结束时，两组在流产、后代死亡率和先天性异常方面未观察到差异。观察到子宫扭转母鼠所生雌性大鼠达到青春期的时间较长，但差异无统计学意义。在后代的脑、内脏或生殖器官中未检测到微观病变。因此，得出的结论是，至少在短期内，子宫扭转母鼠的后代未受影响。总体而言，得出的结论是，这种动物模型适用于子宫扭转和缺血再灌注研究，但不适用于胎儿缺氧研究。
