School of Natural Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
Biomolecular Discovery Research Centre, Macquarie University, North Ryde, NSW 2109, Australia.
Biomolecules. 2023 Oct 20;13(10):1554. doi: 10.3390/biom13101554.
Half of the world's population depends on rice plant cultivation, yet environmental stresses continue to substantially impact the production of one of our most valuable staple foods. The aim of this study was to investigate the changes in the transcriptome of the IAC1131 rice genotype when exposed to a suite of multiple abiotic stresses, either with or without pre-treatment with the plant hormone ABA (Abscisic acid). Four groups of IAC1131 rice plants were grown including control plants incubated with ABA, non-ABA-incubated control plants, stressed plants incubated with ABA, and non-ABA-incubated stressed plants, with leaf samples harvested after 0 days (control) and 4 days (stressed). We found that high concentrations of ABA applied exogenously to the control plants under normal conditions did not alter the IAC1131 transcriptome profile significantly. The observed changes in the transcriptome of the IAC1131 plants in response to multiple abiotic stress were made even more pronounced by ABA pre-treatment, which induced the upregulation of a significant number of additional genes. Although ABA application impacted the plant transcriptome, multiple abiotic stress was the dominant factor in modifying gene expression in the IAC1131 plants. Exogenous ABA application may mitigate the effects of stress through ABA-dependent signalling pathways related to biological photosynthesis functions. Pre-treatment with ABA alters the photosynthesis function negatively by reducing stomatal conductance, therefore helping plants to conserve the energy required for survival under unfavourable environmental conditions.
世界上有一半的人口依赖于水稻种植，但环境压力继续对我们最重要的主食之一的生产产生重大影响。本研究的目的是研究 IAC1131 水稻基因型在受到一系列非生物胁迫时的转录组变化，这些胁迫既可以用植物激素 ABA（脱落酸）预处理，也可以不用。我们种植了四组 IAC1131 水稻植物，包括用 ABA 孵育的对照植物、未用 ABA 孵育的对照植物、用 ABA 孵育的胁迫植物和未用 ABA 孵育的胁迫植物，在 0 天（对照）和 4 天（胁迫）后收获叶片样本。我们发现，在外源施加高浓度 ABA 到正常条件下的对照植物中，不会显著改变 IAC1131 的转录组谱。ABA 预处理使 IAC1131 植物对多种非生物胁迫的转录组变化更加明显，诱导了大量额外基因的上调。尽管 ABA 的应用会影响植物的转录组，但多种非生物胁迫是改变 IAC1131 植物基因表达的主要因素。外源 ABA 的应用可能通过与生物光合作用功能相关的 ABA 依赖信号通路来减轻胁迫的影响。ABA 预处理通过降低气孔导度对光合作用功能产生负面影响，从而帮助植物在不利的环境条件下保存生存所需的能量。
