Laçin Nihat, İzol Bozan Serhat, Özkorkmaz Ebru Gökalp, Deveci Buşra, Tuncer Mehmet Cudi
PhD, Assistant Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Katip Çelebi, İzmir, Turkey. Technical procedures, manuscript preparation and writing, final approval.
PhD, Research Assistant, Department of Periodontology, Faculty of Dentistry, University of Bingöl, Bingöl, Turkey. Technical procedures, manuscript preparation and writing, final approval.
Acta Cir Bras. 2019 Mar 18;34(3):e201900306. doi: 10.1590/s0102-865020190030000006.
To investigate the effects of allopurinol administration on osteoinductive reaction and bone development with graft material.
Thirty-six Wistar albino rats were divided into 3 groups. In the control group, calvarial bone defect was only created without any treatment. In the Defect + Graft group, allograft treatment was performed by forming 8 mm calvarial bone defect. In the Defect + Graft + Allopurinol group, alloplastic bone graft was placed in the calvarial bone defect and then, allopurinol (50 mg/kg/day) treatment was intraperitoneally applied for 28 days.
Histopathological examination revealed inflammation, congestion in the vessels, and an increase in osteoclast cells in the defect area. We also observed that new osteocyte cells, increase in connective tissue fibers, and new bone trabeculae. Osteopontin expression was positive in osteoblast cells and lacunated osteocyte cells were located in the periphery of the new bone trabeculae. Osteopontin expression was also positive in osteoblasts and osteocytes cells of new bone trabeculae in the graft site.
It has been shown that allopurinol treatment in rat calvaria defects may induce osteoblastic activity, matrix development, mature bone cell formation and new bone formation when used with autogenous grafts.
研究给予别嘌醇对移植材料骨诱导反应和骨发育的影响。
将36只Wistar白化大鼠分为3组。对照组仅制造颅骨骨缺损，不进行任何处理。在缺损+移植组，通过制造8毫米颅骨骨缺损进行同种异体移植治疗。在缺损+移植+别嘌醇组，将人工骨移植到颅骨骨缺损处，然后腹腔内给予别嘌醇（50毫克/千克/天）治疗28天。
组织病理学检查显示缺损区域有炎症、血管充血以及破骨细胞增多。我们还观察到有新的骨细胞、结缔组织纤维增加以及新的骨小梁。骨桥蛋白在成骨细胞中表达呈阳性，陷窝状骨细胞位于新骨小梁周边。移植部位新骨小梁的成骨细胞和骨细胞中骨桥蛋白表达也呈阳性。
已表明在大鼠颅骨缺损中使用别嘌醇治疗并结合自体移植时，可能诱导成骨细胞活性、基质发育、成熟骨细胞形成和新骨形成。
