Laçin N, İzol B S, Gökalp Özkorkmaz E, Deveci B, Deveci E
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Katip Çelebi, İzmir, Turkey.
Department of Periodontology, Faculty of Dentistry, University of Bingöl, Bingöl, Turkey.
Folia Morphol (Warsz). 2020;79(3):528-547. doi: 10.5603/FM.a2019.0101. Epub 2019 Sep 6.
This study presents the evaluation of the damage in the bone tissue resulting from a calvarial defect in rats and the efficiency of exposure to an ozone application with an alloplastic bone graft on the calvarial bone damage.
Wistar male rats (n = 56) were divided into four groups: a control group (n = 14), defect and ozone group (n = 14), defect and graft group (n = 14), and defect, graft, and ozone group (n = 14). Under anaesthesia, a circular full-thickness bone defect was created in all groups, and the experimental groups were further divided into two sub-groups, with 7 rats in each group sacrificed at the end of the 4th and 8th weeks. Bone samples were dissected, fixed in 10% formalin solution, and decalcified with 5% ethylene-diamine-tetraacetic acid (EDTA). After the routine follow-up on tissues, immunostaining of osteopontin and osteonectin antibodies was applied to sections and observed under a light microscope.
The control group exhibited osteopontin and osteonectin expression in fibroblasts and inflammatory cells at the end of the 4th week with an acceleration at the 8th week. Ozone administration elucidated new trabecular bone formation by increasing osteoblastic activity. Lastly, our observations underscore that a combination of allograft and ozone application increased the osteoblast, osteocyte, and bone matrix development at the 4th and 8th weeks.
Exposure to an ozone application with an alloplastic bone graft on calvarial bone damage may induce osteoblastic activity, matrix development, mature bone cell formation, and new bone formation in rats.
本研究评估了大鼠颅骨缺损导致的骨组织损伤，以及同种异体骨移植联合臭氧治疗对颅骨损伤的疗效。
将56只雄性Wistar大鼠分为四组：对照组（n = 14）、缺损加臭氧组（n = 14）、缺损加移植组（n = 14）和缺损、移植加臭氧组（n = 14）。在麻醉下，所有组均制作圆形全层骨缺损，实验组进一步分为两个亚组，每组7只大鼠，分别在第4周和第8周结束时处死。解剖骨样本，用10%甲醛溶液固定，并用5%乙二胺四乙酸（EDTA）脱钙。对组织进行常规处理后，对切片进行骨桥蛋白和骨粘连蛋白抗体免疫染色，并在光学显微镜下观察。
对照组在第4周结束时，成纤维细胞和炎性细胞中出现骨桥蛋白和骨粘连蛋白表达，第8周时加速。臭氧治疗通过增加成骨细胞活性促进了新的小梁骨形成。最后，我们的观察结果强调，同种异体移植与臭氧联合应用在第4周和第8周时增加了成骨细胞、骨细胞和骨基质的发育。
在颅骨损伤上应用同种异体骨移植联合臭氧治疗可能会诱导大鼠成骨细胞活性、基质发育、成熟骨细胞形成和新骨形成。
