Yu Xiao-Dong, Yu Xiao-Yun, Fu Ge-Yi, Zhao Zhe, Shen Xia, Sun Cong, Wu Min
1College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
2Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou 310004, PR China.
Int J Syst Evol Microbiol. 2019 May;69(5):1490-1495. doi: 10.1099/ijsem.0.003348. Epub 2019 Mar 20.
A Gram-stain-negative, motile, aerobic, rod-shaped bacterium with flagella, designated ZX-21, was isolated from surface seawater of the East Sea in Zhoushan, China. Growth of strain ZX-21T was observed at 10--35 o°C (optimum, 30 °C), at pH 6.0-8.5 (pHoptimum 6.5-7.0) and in the presence of 0.5-8 % (w/v) NaCl (optimum 3-4 %). It was positive for oxidase and catalase activity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZX-21T constituted an independent lineage within the family Spongiibacteraceae and was most closely related to Zhongshania guokunii (96.83 %). Strain ZX-21T contained ubiquinone-8 (Q-8) as the sole isoprenoid quinone and summed feature 3 (C16 : 1ω77c and/or C16 : 1ω66c), summed feature 8 (C18 : 1ω77c and/or C18 : 1ω66c) and C16 : 0 as the major fatty acids. Phosphatidylglycerol (), phosphatidylethanolamine (), diphosphatidylglycerol () and an unidentified glycolipid were the major cellular polar lipids. The DNA G+C content was 49.1 mol%. Based on itsthe morphological, physiological and chemotaxonomic characteristics, strain ZX-21Tis described as a novel species in a novel genus for whichwith the name Marortus luteolus gen. nov., sp. nov. (type strain ZX-21T = MCCC 1K03431T=KCTC 62160T) is proposed.
从中国舟山东海表层海水中分离出一株革兰氏染色阴性、具鞭毛、需氧、杆状的细菌，命名为ZX-21。菌株ZX-21T在10-35 °C（最适温度30 °C）、pH 6.0-8.5（最适pH 6.5-7.0）以及0.5-8%（w/v）NaCl（最适3-4%）条件下生长。氧化酶和过氧化氢酶活性呈阳性。基于16S rRNA基因序列的系统发育分析表明，菌株ZX-21T在海绵杆菌科内构成一个独立分支，与郭氏中山菌（96.83%）关系最为密切。菌株ZX-21T含有泛醌-8（Q-8）作为唯一的类异戊二烯醌，主要脂肪酸为特征性总和3（C16:1ω7c和/或C16:1ω6c）、特征性总和8（C18:1ω7c和/或C18:1ω6c）以及C16:0。磷脂酰甘油、磷脂酰乙醇胺、二磷脂酰甘油和一种未鉴定的糖脂是主要的细胞极性脂质。DNA G+C含量为49.1 mol%。基于其形态、生理和化学分类特征，菌株ZX-21T被描述为一个新属中的新物种，为此提出新属名为Marortus luteolus gen. nov.，新种名为Marortus luteolus sp. nov.（模式菌株ZX-21T = MCCC 1K03431T = KCTC 62160T）。
