Zhou Shun, Ren Qiaomeng, Li Yuying, Liu Jiwen, Wang Xiaolei, Wu Yanhong, Zhang Yulin, Zhang Xiao-Hua
1College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
2Laboratory for Marine Ecology and Environmental Science, National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3424-3429. doi: 10.1099/ijsem.0.002999. Epub 2018 Sep 11.
A Gram-stain-negative, strictly aerobic, short-rod-shaped bacterium, motile by a single polar flagellum, designated OUC007, was isolated from seawater at a depth of 1000 m in the Mariana Trench. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain OUC007 formed a lineage within the family Salinisphaeraceae that was distinct from the most closely related genera Oceanococcus and Salinisphaera, with 16S rRNA gene sequences similarities ranging from 89.3 to 90.8 %, respectively. Strain OUC007 grew with 0-7 % (w/v) NaCl (optimum, 2-3 %), at 10-45 °C (37 °C) and at pH 7.0-9.0 (7.0-8.0). The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids of strain OUC007 comprised one phosphatidylethanolamine and three unidentified polar lipids. The predominant fatty acid (more than 10 % of total fatty acids) was summed feature 8 (C18 : 1ω7c or/and C18 : 1ω6c). The DNA G+C content of strain OUC007 was 63.1 mol%. On the basis of polyphasic taxonomic analysis, the strain OUC007 is considered to represent a novel species in a new genus of the family Salinisphaeraceae, for which the name Abyssibacter profundi gen. nov., sp. nov. is proposed. The type strain is OUC007 (=KCTC 52933=MCCC 1K03450).
从马里亚纳海沟1000米深处的海水中分离出一株革兰氏染色阴性、严格需氧、短杆状、具单根极鞭毛运动的细菌，命名为OUC007。基于16S rRNA基因序列的系统发育分析表明，菌株OUC007在盐球菌科内形成了一个分支，与最相近的属海洋球菌属和盐球菌属不同，其16S rRNA基因序列相似性分别为89.3%至90.8%。菌株OUC007在含0 - 7%（w/v）NaCl（最适为2 - 3%）、10 - 45℃（37℃）和pH 7.0 - 9.0（7.0 - 8.0）的条件下生长。主要呼吸醌为泛醌-8（Q - 8）。菌株OUC007的极性脂质包括一种磷脂酰乙醇胺和三种未鉴定的极性脂质。主要脂肪酸（占总脂肪酸的10%以上）为总和特征8（C18:1ω7c或/和C18:1ω6c）。菌株OUC007的DNA G + C含量为63.1 mol%。基于多相分类分析，菌株OUC007被认为代表盐球菌科一个新属中的一个新物种，为此提出新属名为深渊杆菌属（Abyssibacter gen. nov.），新种名为深渊深渊杆菌（Abyssibacter profundi sp. nov.）。模式菌株为OUC007（=KCTC 52933 = MCCC 1K03450）。
