Sandoval Regina Faye C, Cumagun Christian Joseph R
Institute of Weed Science, Entomology and Plant Pathology, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna 4031, Philippines.
Molecular Phytopathology and Mycotoxin Research, University of Göttingen, Grisebachstrasse 6, 37077 Göttingen, Germany.
Microorganisms. 2019 Mar 19;7(3):88. doi: 10.3390/microorganisms7030088.
Forty-two isolates were collected from rice, mung bean, and grasses from Laguna, Philippines. Sixteen isolates were binucleate (BNR), while 26 were multinucleate (MNR). BNR isolates produced white to brown, small sclerotia (<1.0 mm) except for mung bean isolates. Twenty MNR isolates produced big (>1.0 mm), light to dark brown sclerotia, three produced salmon-colored masses in the medium, and three did not produce sclerotia. Twenty-three MNR isolates were identified as AG1-IA using specific primers. Deduced Internal Transcribed Spacer (ITS) sequences of BNR isolates D1FL, NVL, and ScNL shared 100, 97, and 100% identity with , respectively, while MNR isolates BMgL, IbMgL, and MaSL that produced salmon-colored masses shared 100, 90, and 100% identity with , respectively. Preliminary analysis of the DNA fingerprint patterns generated by repetitive-element PCR (rep-PCR) clustered the 42 isolates into three: and , together with sp isolates were pathogenic on rice (TN1), barnyard grass, mungbean (Pagasa 3), and tomato (Athena), while and isolates were only pathogenic on rice, , and tomato. and were found to be more virulent than
从菲律宾拉古纳的水稻、绿豆和禾本科植物中收集了42个分离株。16个分离株为双核（BNR），26个为多核（MNR）。除绿豆分离株外，BNR分离株产生白色至棕色的小菌核（<1.0毫米）。20个MNR分离株产生大的（>1.0毫米）、浅棕色至深棕色的菌核，3个在培养基中产生鲑鱼色团块，3个不产生菌核。使用特异性引物将23个MNR分离株鉴定为AG1-IA。BNR分离株D1FL、NVL和ScNL的推导内部转录间隔区（ITS）序列分别与 具有100%、97%和100%的同一性，而产生鲑鱼色团块的MNR分离株BMgL、IbMgL和MaSL分别与 具有100%、90%和100%的同一性。对重复元件PCR（rep-PCR）产生的DNA指纹图谱进行初步分析，将42个分离株聚类为三类： 和 ， 与 分离株对水稻（TN1）、稗草、绿豆（Pagasa 3）和番茄（雅典娜）致病，而 和 分离株仅对水稻、 和番茄致病。发现 和 比 更具毒性
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