Bioproducts Department, Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Km 14 vía Bogotá a Mosquera, Mosquera, Colombia.
Agricultural Microbiology Laboratory, Tibaitatá Research Center, Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Km 14 vía Bogotá a Mosquera, Mosquera, Colombia.
World J Microbiol Biotechnol. 2024 Feb 26;40(4):108. doi: 10.1007/s11274-024-03897-0.
The incorporation of biological control agents (BCAs) such as Trichoderma spp. in agricultural systems favors the transition towards sustainable practices of plant nutrition and diseases control. Novel bioproducts for crop management are called to guarantee sustainable antagonism activity of BCAs and increase the acceptance of the farmers. The encapsulation in polymeric matrices play a prominent role for providing an effective carrier/protector and long-lasting bioproduct. This research aimed to study the influence of biopolymer in hydrogel capsules on survival and shelf-life of T. koningiopsis. Thus, two hydrogel capsules prototypes based on alginate (P1) and amidated pectin (P2), containing conidia of T. koningiopsis Th003 were formulated. Capsules were prepared by the ionic gelation method and calcium gluconate as crosslinker. Conidia releasing under different pH values of the medium, survival of conidia in drying capsules, storage stability, and biocontrol activity against rice sheath blight (Rhizoctonia solani) were studied. P2 prototype provided up to 98% survival to Th003 in fluid bed drying, faster conidia releasing at pH 5.8, storage stability greater than 6 months at 18 °C, and up to 67% of disease reduction. However, both biopolymers facilitate the antagonistic activity against R. solani, and therefore can be incorporated in novel hydrogel capsules-based biopreparations. This work incites to develop novel biopesticides-based formulations with potential to improve the delivery process in the target site and the protection of the active ingredient from the environmental factors.
将生物防治剂（BCAs）如木霉属（Trichoderma spp.）纳入农业系统有利于向植物营养和疾病控制的可持续实践转变。新型生物制品用于作物管理，以保证 BCAs 的可持续拮抗活性，并提高农民的接受度。在聚合物基质中的包封对于提供有效的载体/保护剂和持久的生物制品起着突出的作用。本研究旨在研究生物聚合物在水凝胶胶囊中对金龟子木霉（T. koningiopsis）存活和保质期的影响。因此，基于海藻酸钠（P1）和酰胺化果胶（P2）制备了两种水凝胶胶囊原型，其中包含金龟子木霉 Th003 的分生孢子。通过离子凝胶化法和葡萄糖酸钙作为交联剂制备胶囊。研究了在不同 pH 值的培养基中释放分生孢子、干燥胶囊中分生孢子的存活、储存稳定性以及对水稻纹枯病（Rhizoctonia solani）的生物防治活性。P2 原型在流化床干燥中为 Th003 提供了高达 98%的存活率，在 pH 值为 5.8 时更快地释放分生孢子，在 18°C 下储存稳定性超过 6 个月，病害减轻率高达 67%。然而，这两种生物聚合物都有利于对 R. solani 的拮抗活性，因此可以纳入新型基于水凝胶胶囊的生物制剂中。这项工作激发了开发新型生物农药制剂的研究，这些制剂有可能改善目标部位的输送过程，并保护有效成分免受环境因素的影响。
