Johanson A, Turner H C, McKay G J, Brown A E
Natural Resources Institute, University of Greenwich, Chatham, Kent, UK.
FEMS Microbiol Lett. 1998 May 15;162(2):289-94. doi: 10.1111/j.1574-6968.1998.tb13011.x.
Identification of Rhizoctonia solani, R. oryzae and R. oryzae-sativae, components of the rice sheath disease complex, is extremely difficult and often inaccurate and as a result may hinder the success of extensive breeding programmes throughout Asia. In this study, primers designed from unique regions within the rDNA internal transcribed spacers have been used to develop a rapid PCR-based diagnostic test to provide an accurate identification of the species on rice. Tests on the specificity of the primers concerned showed that they provide the means for accurate identification of the Rhizoctonia species responsible for sheath diseases in rice.
水稻纹枯病复合体的组成部分，即立枯丝核菌、稻梨孢菌和稻-稻梨孢菌，极难鉴定，而且往往不准确，因此可能会阻碍亚洲各地大规模育种计划的成功。在本研究中，从核糖体DNA内部转录间隔区的独特区域设计的引物已被用于开发一种基于PCR的快速诊断测试，以准确鉴定水稻上的这些物种。对相关引物特异性的测试表明，它们为准确鉴定引起水稻纹枯病的丝核菌物种提供了方法。
