Mote Marine Laboratory, Marine Immunology Program, 1600 Ken Thompson Parkway, Sarasota, FL, 34236, USA.
Marine Science Center, Northeastern University, 430 Nahant Road, Nahant, Massachusetts, 01908, USA.
Sci Rep. 2019 Mar 20;9(1):4892. doi: 10.1038/s41598-019-39316-w.
The need for sustainable bacterial management approaches in aquaculture is crucial for advancement of the industry. Probiotics are a promising strategy as evidenced by benefits demonstrated in intensive larviculture of various marine fish species. In this study we investigate the effects of a mixed Bacillus species (B. licheniformis and B. amyloliquefaciens) probiotic on rearing of larval common snook (Centropomus undecimalis). Experimental treatments included (1) probiotics supplemented to the water and live feed, (2) probiotics supplemented to the water only, and (3) no probiotic controls. Data from two separate trials indicated up to 2.5 times higher survival with probiotic addition, as well as 20% higher survival 7 days following a transport event. These benefits were not explained by faster growth, measured water quality parameters, or innate immune enzyme activities. Microbiota analysis indicated the importance of system stabilization prior to larval stocking to improve rearing success and probiotic performance. ied Potential probiotic benefits include accelerated gastrointestinal tract development, enhanced immunity, inhibition of opportunistic bacteria, and improvements to water quality parameters. Results suggest this probiotic should be tested in other marine fish species in order to reduce larval rearing bottlenecks.
在水产养殖中，需要可持续的细菌管理方法，这对于推动该行业的发展至关重要。益生菌是一种很有前途的策略，因为它在各种海水鱼的集约化育苗中表现出了益处。在这项研究中，我们研究了混合芽孢杆菌（地衣芽孢杆菌和解淀粉芽孢杆菌）益生菌对幼年美洲海鲢（Centropomus undecimalis）养殖的影响。实验处理包括：（1）向水和活饲料中添加益生菌，（2）仅向水中添加益生菌，（3）不添加益生菌对照。来自两个独立试验的数据表明，添加益生菌可将存活率提高高达 2.5 倍，并且在运输事件发生后 7 天，存活率提高 20%。这些益处不能用更快的生长、测量的水质参数或固有免疫酶活性来解释。微生物组分析表明，在进行鱼苗放养之前，系统的稳定化对于提高养殖成功率和益生菌的性能至关重要。有潜力的益生菌益处包括加速胃肠道发育、增强免疫力、抑制机会性细菌以及改善水质参数。结果表明，应该在其他海水鱼类中测试这种益生菌，以减少鱼苗养殖的瓶颈。
