Ghori Ifra, Tubassam Misbah, Ahmad Tanveer, Zuberi Amina, Imran Muhammad
Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Biotechnology, Fatima Jinnah Women University, Rawalpindi, Pakistan.
Front Physiol. 2022 Sep 9;13:949559. doi: 10.3389/fphys.2022.949559. eCollection 2022.
The current study was targeted to determine the effect of probiotics on the growth, physiology, and gut microbiology of fingerlings. One hundred and twenty fishes were divided into four dietary groups, each in triplicate for a feeding trial of 90 days. These treatments included T0 (control, basal diet) used as the reference, and three probiotic-supplemented diets represented as Tbc (), Tgc (), and Tmc ( and ). The probiotics were supplemented at a level of 1 × 10 CFU/g feed. Fishes nurtured on probiotic-added diet showed significantly high physiological improvement ( < 0.05) in terms of growth, feed utilization capacity, hematological profile, and digestive enzymes as compared to control. The fish were subjected to a challenge test after a 90-day feeding trial. The Tmc exhibited maximum fish growth when challenged by and showed fish survival when compared to control, in which fish mortality was examined. Fish gut microbial composition was modulated by probiotic treatments, especially in Tgc and Tmc as compared to control. The absence of opportunistic pathogens such as and and detection of lower levels of and in treated groups indicate the gut modulation driven by applied probiotics. The QAUGC01 was retrieved in yeast metagenomics data, which might be due to the production of polyamines by them that facilitated adherence and consequent persistence. In conclusion, it can be suggested that the probiotic-supplemented diet could enhance fish growth and feed efficiency through community modulation and digestive enzymes, which could be a milestone in local aquaculture.
本研究旨在确定益生菌对鱼种生长、生理和肠道微生物群的影响。120条鱼被分为四个饮食组，每组一式三份，进行为期90天的饲养试验。这些处理包括用作对照的T0（基础日粮），以及三种添加益生菌的日粮，分别表示为Tbc（）、Tgc（）和Tmc（和）。益生菌的添加水平为1×10CFU/g饲料。与对照组相比，食用添加益生菌日粮的鱼在生长、饲料利用能力、血液学指标和消化酶方面表现出显著更高的生理改善（<0.05）。在90天的饲养试验后，对鱼进行了攻毒试验。在受到攻击时，Tmc组的鱼生长最快，与对照组相比，该组鱼的存活率更高，对照组检查了鱼的死亡率。益生菌处理调节了鱼的肠道微生物组成，尤其是与对照组相比，Tgc组和Tmc组更为明显。处理组中没有和等机会性致病菌，且和的水平较低，这表明应用益生菌驱动了肠道调节。在酵母宏基因组数据中检索到了QAUGC01，这可能是由于它们产生了多胺，促进了黏附并使其得以持续存在。总之，可以认为添加益生菌的日粮可以通过群落调节和消化酶来提高鱼的生长和饲料效率，这可能是当地水产养殖的一个里程碑。
