Endrinaldi Endrinaldi, Darwin Eryati, Zubir Nasrul, Revilla Gusti
Postgraduate Biomedical Science, Faculty of Medicine, Andalas University, Padang, Indonesia.
Department of Chemistry, Faculty of Medicine, Andalas University, Padang, Indonesia.
Open Access Maced J Med Sci. 2019 Feb 27;7(4):529-535. doi: 10.3889/oamjms.2019.152. eCollection 2019 Feb 28.
Osteoarthritis (OA) is generally considered a degenerative joint disease caused by biomechanical changes and the ageing process. In OA pathogenesis, the development of OA is thought to be regulated largely by excess matrix metalloproteinase (MMP), which contributes to the degradation of extracellular matrices such as MMP-1 and Interleukin-4.
This study aims to prove the influence of Mesenchymal Stem Cell Wharton Jelly on decreasing MMP-1 levels and increasing IL-4 which is a specific target as a target component in cases of osteoarthritis in vivo.
This research is an experimental study with the design of Post-Test-Only Control Group Design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the third week after MIA induction. The serum MMP-1 and IL-4 levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. The value of p < 0.05 was said to be statistically significant.
The result showed that serum MMP-1 levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05). Serum IL-4 levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05).
This study concluded that MSC-WJ increased MMP-1 levels and IL-4 levels in serum OA rats. MSC-WJ showed a negative effect on MMP-1 in the serum of OA rats.
骨关节炎（OA）通常被认为是一种由生物力学变化和衰老过程引起的退行性关节疾病。在OA发病机制中，OA的发展被认为主要受过量基质金属蛋白酶（MMP）的调节，MMP会导致细胞外基质如MMP - 1和白细胞介素 - 4的降解。
本研究旨在证明间充质干细胞华通氏胶对降低MMP - 1水平和增加IL - 4的影响，IL - 4是体内骨关节炎病例中作为靶标成分的特定靶点。
本研究是一项实验性研究，采用仅后测对照组设计。样本包括16只OA大鼠作为对照组和16只接受间充质干细胞华通氏胶治疗的OA大鼠作为治疗组。通过向右膝关节腔内注射碘乙酸钠（MIA）诱导OA。在MIA诱导后的第三周给予间充质干细胞华通氏胶。使用酶联免疫吸附测定（ELISA）方法在接受间充质干细胞华通氏胶治疗3周后测量血清MMP - 1和IL - 4水平。使用的统计检验是独立样本t检验。p值<0.05被认为具有统计学意义。
结果显示，接受间充质干细胞华通氏胶治疗的组血清MMP - 1水平高于对照组（p<0.05）。接受间充质干细胞华通氏胶治疗的组血清IL - 4水平高于对照组（p<0.05）。
本研究得出结论，间充质干细胞华通氏胶增加了血清OA大鼠的MMP - 1水平和IL - 4水平。间充质干细胞华通氏胶对OA大鼠血清中的MMP - 1显示出负面影响。
