Endrinaldi Endrinaldi, Darwin Eryati, Zubir Nasrul, Revilla Gusti
Doctoral Student of Postgraduate Biomedical Science, Faculty of Medicine, Andalas University, Padang, Indonesia.
Department of Chemistry, Faculty of Medicine, Andalas University, Padang, Indonesia.
Open Access Maced J Med Sci. 2019 Apr 29;7(8):1270-1275. doi: 10.3889/oamjms.2019.155. eCollection 2019 Apr 30.
Osteoarthritis (OA) is one of the most common diseases among the elderly. OA occurs due to an imbalance between degradation and synthesis in articular joint tissue, causing changes in joint components such as cells, matrices and molecular production. Therefore, knowledge of cartilage-degrading enzymes such as ADAMTS-4 and iNOS is needed.
This study aims to prove the effect of Mesenchymal Stem Cell Wharton Jelly on decreasing ADAMTS-4 levels as cartilage-degrading enzymes and increasing levels of iNOS which showed the immunosuppressive potential of MSC-WJ in cases of osteoarthritis in vivo.
This research is an experimental study with the design of Post-test-Only Control Group Design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the third week after MIA induction. The serum ADAMTS-4 and iNOS levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. The value of p < 0.05 was said to be statistically significant.
The result showed that serum ADAMTS-4 levels were lower in the group treated with MSC-WJ than in the control group, but not statistically significant (p > 0.05). Serum iNOS levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05).
This study concluded that MSC-WJ significantly reduced ADAMTS-4 levels and increased the serum iNOS levels of OA rats.
骨关节炎（OA）是老年人中最常见的疾病之一。OA是由于关节组织中降解与合成之间的失衡所致，会引起关节成分如细胞、基质和分子生成的变化。因此，需要了解诸如ADAMTS-4和诱导型一氧化氮合酶（iNOS）等软骨降解酶。
本研究旨在证明间充质干细胞华通氏胶（MSC-WJ）对降低作为软骨降解酶的ADAMTS-4水平以及提高iNOS水平的作用，iNOS水平表明了MSC-WJ在体内骨关节炎病例中的免疫抑制潜力。
本研究是一项实验性研究，采用仅后测对照组设计。样本包括16只OA大鼠作为对照组和16只接受MSC-WJ治疗的OA大鼠作为治疗组。通过向右膝关节腔内注射碘乙酸钠（MIA）来诱导OA。在MIA诱导后的第三周给予MSC-WJ。使用酶联免疫吸附测定（ELISA）法在接受MSC-WJ治疗3周后测量血清ADAMTS-4和iNOS水平。所使用的统计检验是独立样本t检验。p值<0.05被认为具有统计学意义。
结果显示，接受MSC-WJ治疗的组中血清ADAMTS-4水平低于对照组，但无统计学意义（p>0.05）。接受MSC-WJ治疗的组中血清iNOS水平高于对照组（p<0.05）。
本研究得出结论，MSC-WJ显著降低了OA大鼠的ADAMTS-4水平并提高了血清iNOS水平。
