Effect of deletion of the gene on selected virulence of .

BACKGROUND/PURPOSE: The most potent virulence factors of the periodontal pathogen are gingipains, three cysteine proteases (RgpA, RgpB, and Kgp) that bind and cleave a wide range of host proteins. Considerable proof indicates that RgpA contributes to the entire virulence of the organism and increases the risk of periodontal disease by disrupting the host immune defense and destroying the host tissue. However, the functional significance of this proteinase is incompletely understood. It is important to analyze the effect of arginine-specific gingipain A gene () on selected virulence and physiological properties of .

MATERIALS AND METHODS

Electroporation and homologous recombination were used to construct an mutant of ATCC33277. The mutant was verified by polymerase chain reaction and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cell structures of the mutant were examined by transmission electron microscopy and homotypic biofilm formation was examined by confocal laser scanning microscopy.

RESULTS

Gene analysis revealed that the gene was deleted and replaced by a drug resistance gene marker. The defect of the gene resulted in a complete loss of RgpA proteinase, a reduction of out membrane vesicles and hemagglutination, and an increase in homotypic biofilm formation.

CONCLUSION

Our data indicate that an gene deficient strain of is successfully isolated. RgpA may have a variety of physiological and pathological roles in .