Hsiao Yu-Feng, Yang Li-Chiu, Chou Yu-Shian, Ho Ya-Ping, Lin Ying-Chu, Ho Kun-Yen, Wu Yi-Min, Tsai Chi-Cheng
Institute of Biomedical Nutrition, Hungkuang University, Taichung, Taiwan.
Periodontics, University Hospital and College of Oral Medicine, Chung Shan Medical University, Taichung, Taiwan.
J Dent Sci. 2016 Dec;11(4):411-418. doi: 10.1016/j.jds.2016.07.001. Epub 2016 Aug 9.
BACKGROUND/PURPOSE: Matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs (TIMPs) have been shown to play an important role in the pathogenesis of tissue destruction in periodontitis. The associations between single nucleotide polymorphisms (SNPs) in the promoter regions of MMP-2, MMP-9, and TIMP-2 genes and the risk of aggressive periodontitis (AgP) and chronic periodontitis (CP) were investigated in a Taiwanese population.
MMP-2 C-1306T, C-735T, T-790G, and MMP-9 C-1562T and TIMP-2 G-418C SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis in 69 patients and 129 patients with AgP and CP, respectively, and 117 periodontal healthy individuals who served as healthy controls (HC). Chi-square test and logistic regression analysis were used to investigate the possible association of genotypes with periodontitis.
No significant differences in the distributions of the C-1306T and C-735T variants between periodontitis and HC were detected. Patients with genotype of MMP-2 -790 TT or T allele of MMP-2-790T/G as compared to genotypes of GT + GG genotypes or G allele, were less susceptible to CP [odds ratio (OR) = 0.50, 95% confidence interval (CI) = 0.25-1.00 and OR = 0.52, 95% CI = 0.28-0.96, respectively]. The frequencies of TIMP-2 G-418C gene polymorphisms in nonsmokers were statistically significantly different among AgP, CP, and HC groups (P = 0.024). The nonalcohol drinking participants with C allele of MMP-9 C-1562T as compared to T allele, were less susceptible to AgP (adjusted OR = 0.4; 95% CI, 0.18-0.90).
It is suggested that MMP-2 T-790G, MMP-9 C-1562T, and TIMP-2 G-418C gene polymorphisms might be associated with periodontitis in the Taiwanese Han population.
背景/目的：基质金属蛋白酶（MMPs）和基质金属蛋白酶组织抑制剂（TIMPs）已被证明在牙周炎组织破坏的发病机制中起重要作用。本研究在台湾人群中调查了MMP-2、MMP-9和TIMP-2基因启动子区域的单核苷酸多态性（SNPs）与侵袭性牙周炎（AgP）和慢性牙周炎（CP）风险之间的关联。
分别采用聚合酶链反应-限制性片段长度多态性分析对69例AgP患者、129例CP患者以及117名牙周健康个体（作为健康对照，HC）进行MMP-2 C-1306T、C-735T、T-790G，MMP-9 C-1562T和TIMP-2 G-418C SNPs基因分型。采用卡方检验和逻辑回归分析来研究基因型与牙周炎之间的可能关联。
未检测到牙周炎患者与HC之间C-1306T和C-735T变异分布的显著差异。与GT + GG基因型或G等位基因相比，MMP-2 -790 TT基因型或MMP-2-790T/G的T等位基因患者对CP的易感性较低[比值比（OR）= 0.50，95%置信区间（CI）= 0.25 - 1.00；OR = 0.52，95% CI = 0.28 - 0.96]。非吸烟者中TIMP-2 G-418C基因多态性的频率在AgP、CP和HC组之间存在统计学显著差异（P = 0.024）。与T等位基因相比，MMP-9 C-1562T的C等位基因非饮酒参与者对AgP的易感性较低（校正OR = 0.4；95% CI，0.18 - 0.90）。
提示MMP-2 T-790G、MMP-9 C-1562T和TIMP-2 G-418C基因多态性可能与台湾汉族人群的牙周炎有关。
