Sarkar Puja, Muthuraj Thamil Selvan, Bandyopadhyay Prasanta, Ghosh Papita
Dental Department, North Bengal Medical College and Hospital, Siliguri, West Bengal, India.
Department of Periodontics, CSI College of Dental Sciences and Research, Madurai, Tamil Nadu, India.
J Indian Soc Periodontol. 2022 Jul-Aug;26(4):348-352. doi: 10.4103/jisp.jisp_80_21. Epub 2022 Jul 2.
Matrix metalloproteinases (MMPs) are a major group of enzymes, released in inflamed periodontal tissues in large quantities, resulting in connective tissue matrix breakdown. One of the most predominant MMPs is MMP-9. Association between chronic periodontitis (CP) and MMP-9 gene polymorphism (GP) in some ethnic populations has been already established. The aim of the current study was to assess the association of single-nucleotide polymorphism in the promoter region of MMP-9 gene with CP in Kolkata population, if any.
Forty eligible individuals were recruited for the study and grouped as A and B: Group A (twenty periodontally healthy individuals) and Group B (twenty CP patients). Venous blood samples collected from all the forty individuals were used to isolate the genomic DNA. GP for MMP-9 gene was detected using polymerase chain reaction and sequencing method. The data obtained were then analyzed statistically.
A significantly different genotypic and allelic variation was observed in the control and test groups, with C/T genotype and T allele present in only CP group.
Within the limitations of this study, we can conclude that there was no significant association between patients with periodontitis and MMP-9-1562 C/T promoter GP.
基质金属蛋白酶（MMPs）是一类主要的酶，在炎症性牙周组织中大量释放，导致结缔组织基质分解。最主要的MMPs之一是MMP-9。一些种族人群中慢性牙周炎（CP）与MMP-9基因多态性（GP）之间的关联已经确立。本研究的目的是评估加尔各答人群中MMP-9基因启动子区域单核苷酸多态性与CP之间是否存在关联。
招募40名符合条件的个体进行研究，并分为A组和B组：A组（20名牙周健康个体）和B组（20名CP患者）。从所有40名个体采集的静脉血样本用于分离基因组DNA。使用聚合酶链反应和测序方法检测MMP-9基因的GP。然后对获得的数据进行统计学分析。
在对照组和试验组中观察到显著不同的基因型和等位基因变异，C/T基因型和T等位基因仅存在于CP组中。
在本研究的局限性内，我们可以得出结论，牙周炎患者与MMP-9 - 1562 C/T启动子GP之间没有显著关联。
