Anderson Richard A, Wallace W Hamish B, Telfer Evelyn E
Medical Research Council, Centre for Reproductive Health, Queen's Medical Research Institute, 47 Little France Crescent, EdinburghEH16 4TJ, UK.
Department of Haematology/Oncology, Royal Hospital for Sick Children, 9 Sciennes Rd, Edinburgh EH9 1LF, UK.
Hum Reprod Open. 2017 Mar 29;2017(1):hox001. doi: 10.1093/hropen/hox001. eCollection 2017.
Small case series have reported successful live births after ovarian tissue cryopreservation and orthotopic transplantation, demonstrating that it can be of value in increasing the chance of successful pregnancy after treatment for cancer and other fertility-impacting diseases in adult women.
This review is intended to set out the current clinical issues in the field of ovarian tissue cryopreservation, and elucidate the status of laboratory studies to address these.
We reviewed the English-language literature on ovarian tissue cryopreservation and in vitro maturation (IVM) of ovarian follicles.
Ovarian tissue cryopreservation is increasingly used for fertility preservation and, whilst areas for development remain (optimal patient selection, minimizing risk of contamination by malignant cells and IVM protocols), there are emerging data as to its efficacy. We review the current status of ovarian tissue cryopreservation in girls and young women facing loss of fertility from treatment of cancer and other serious diseases. Increasingly large cohort studies are reporting on success rates from ovarian tissue cryopreservation giving an indication of likely success rates. Patient selection is necessary to ensure the safety and effectiveness of this approach, especially in the very experimental situation of its application to prepubertal girls. There are continuing developments in supporting follicle development .
The evidence base consists largely of case series and cohort studies, thus there is the possibility of bias in key outcomes. development of human ovarian follicles remains some way from clinical application.
Ovarian tissue cryopreservation is becoming established as a valuable approach to the preservation of fertility in women. Its application in prepubertal girls may be of particular value, as it offers the only approach in this patient group. For both girls and young women, more accurate data are needed on the likelihood of successful childbirth after this procedure and the factors that underpin successful application of this approach, which will lead to its more effective use.
STUDY FUNDING/COMPETING INTERESTS: The author's work in this field is supported by Medical Research Grant (MRC) grants G0901839 and MR/L00299X/1 and partially undertaken in the MRC Centre for Reproductive Health which is funded by MRC Centre grant MR/N022556/1. The authors declare that there is no conflict of interest that could prejudice the impartiality of the present research.
小规模病例系列报道了卵巢组织冷冻保存及原位移植后成功分娩的案例，表明其在提高成年女性癌症及其他影响生育疾病治疗后成功妊娠几率方面具有价值。
本综述旨在阐述卵巢组织冷冻保存领域当前的临床问题，并阐明针对这些问题的实验室研究现状。
我们检索了关于卵巢组织冷冻保存及卵巢卵泡体外成熟（IVM）的英文文献。
卵巢组织冷冻保存越来越多地用于生育力保存，尽管仍有需要发展的领域（最佳患者选择、将恶性细胞污染风险降至最低以及IVM方案），但关于其疗效的新数据不断涌现。我们综述了面临因癌症及其他严重疾病治疗而丧失生育力的女孩和年轻女性中卵巢组织冷冻保存的现状。越来越多的大型队列研究报告了卵巢组织冷冻保存的成功率，给出了可能的成功率指标。患者选择对于确保该方法的安全性和有效性至关重要，尤其是在将其应用于青春期前女孩这种非常实验性的情况下。支持卵泡发育方面不断有进展。
证据基础主要由病例系列和队列研究组成，因此关键结果可能存在偏差。人类卵巢卵泡的发育距离临床应用仍有一段路要走。
卵巢组织冷冻保存正逐渐成为一种有价值的女性生育力保存方法。其在青春期前女孩中的应用可能具有特殊价值，因为这是该患者群体的唯一方法。对于女孩和年轻女性而言，都需要更准确的数据来了解此程序后成功分娩的可能性以及支撑该方法成功应用的因素，这将使其得到更有效的利用。
研究资金/利益冲突：作者在该领域的工作得到了医学研究基金（MRC）的资助，资助编号分别为G0901839和MR/L00299X/1，部分工作在由MRC中心资助MR/N022556/1的MRC生殖健康中心进行。作者声明不存在可能损害本研究公正性的确利益冲突。
