长链非编码 RNA NRON 通过促进 NFATc3 磷酸化缓解心房纤维化。
LncRNA NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation.
机构信息
Department of Cardiac Intensive Care Unit, CICU, The First Affliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, People's Republic of China.
Thoracic Surgery, Cardiacmacrovascular Surgery, Shaoxing people's Hospital, Shaoxing, 312000, Zhejiang, People's Republic of China.
出版信息
Mol Cell Biochem. 2019 Jul;457(1-2):169-177. doi: 10.1007/s11010-019-03521-y. Epub 2019 Mar 20.
The aim of this study was to explore the role of NRON in the atrial fibrosis. The expression of NRON in atrial tissue was detected using qRT-PCR. The protein levels of collagen I, collagen III, NFATc3 and p-NFATc3 were determined by western blot. Immunohistochemistry assay were performed to observe expression and distribution of collagen I in atrial tissues. The atrial fibroblasts were authenticated by vimentin/troponin immunofluorescence staining. Fibroblast proliferation was detected by CCK-8 assay. The morphological changes of cardiac tissue were observed by HE staining. Myocardial fibrosis was detected by masson staining. NRON expression was significantly downregulated in atrial tissues of AF. NRON suppressed fibroblast proliferation; expression of collagen I and collagen III; activation of NFATc3 and nucleu import. NRON promoted p-NFATc3 expression and alleviated atrial fibrosis in vivo. Our data indicated that NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation.
本研究旨在探讨 NRON 在心房纤维化中的作用。通过 qRT-PCR 检测 NRON 在心房组织中的表达。通过 Western blot 测定胶原 I、胶原 III、NFATc3 和 p-NFATc3 的蛋白水平。免疫组织化学法观察心房组织中胶原 I 的表达和分布。通过波形蛋白/肌钙蛋白免疫荧光染色鉴定心房成纤维细胞。通过 CCK-8 测定法检测成纤维细胞增殖。通过 HE 染色观察心肌组织的形态变化。通过 Masson 染色检测心肌纤维化。AF 患者心房组织中 NRON 表达明显下调。NRON 抑制成纤维细胞增殖；胶原 I 和胶原 III 的表达；NFATc3 的激活和核内易位。NRON 促进 p-NFATc3 的表达，减轻体内心房纤维化。我们的数据表明，NRON 通过促进 NFATc3 磷酸化来减轻心房纤维化。
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