Lieberman M M, Allen R C
Can J Microbiol. 1986 Jun;32(6):531-3. doi: 10.1139/m86-098.
The opsonic capacity of antisera to Pseudomonas aeruginosa ribosomal vaccine fractions was determined by a chemiluminescent technique. Antiserum to a vaccine fraction ("peak A") containing lipopolysaccharide (antiserum A), and antiserum to a vaccine fraction ("peak B"), which did not contain detectable amounts of lipopolysaccharide (antiserum B), were used to opsonify live or formalin-treated bacteria. Polymorphonuclear leukocytes were then stimulated by the opsonified bacteria in the presence of the chemiluminigenic probe, luminol, resulting in the observed chemiluminescence. The data obtained indicated that the antisera had comparable opsonic activity with live (untreated) bacteria. However, antiserum B had far less opsonic activity than did antiserum A when formalinized bacteria were used. Owing to the effects of formaldehyde on protein, these results were interpreted as evidence to suggest that the opsonic activities of the two antisera are dependent on different antigens on the bacterial cell surface. Antiserum A activity is probably dependent on lipopolysaccharide to a great extent, whereas antiserum B activity is most likely dependent primarily on a protein(s).
采用化学发光技术测定了抗铜绿假单胞菌核糖体疫苗组分血清的调理吞噬能力。用针对含脂多糖的疫苗组分（“A峰”）的抗血清（抗血清A）和针对不含可检测量脂多糖的疫苗组分（“B峰”）的抗血清（抗血清B）对活细菌或经福尔马林处理的细菌进行调理。然后在化学发光探针鲁米诺存在的情况下，用经调理的细菌刺激多形核白细胞，从而产生观察到的化学发光。获得的数据表明，抗血清对活的（未处理的）细菌具有相当的调理活性。然而，当使用经福尔马林处理的细菌时，抗血清B的调理活性远低于抗血清A。由于甲醛对蛋白质的影响，这些结果被解释为表明两种抗血清的调理活性依赖于细菌细胞表面不同抗原的证据。抗血清A的活性可能在很大程度上依赖于脂多糖，而抗血清B的活性最可能主要依赖于一种或多种蛋白质。
