Johnson W, Pesanti E, Elliott J
Infect Immun. 1979 Nov;26(2):698-704. doi: 10.1128/iai.26.2.698-704.1979.
A high-molecular-weight surface component (F-1 fraction) has been isolated from the four serogroups of Legionella pneumophila. Antibody raised against live organisms was found by microagglutination assay to be specific for the homologous serogroup. Agglutinating activity of antiserum was markedly diminished after absorption with the homologous, but not heterologous, F-1 fraction. In addition, it was found that L. pneumophila organisms were not interiorized by rat alveolar macrophages or mouse peritoneal macrophages in the absence of antiserum, whereas homologous antiserum effectively opsonized the organisms. The opsonizing activity of serogroup-specific antisera was eliminated by absorption of the antisera with the homologous, but not heterologous, F-1 fraction. These data indicate that the serogroup-specific antigen of L. pneumophila resides in the F-1 fraction and that antibody to the F-1 fraction is required for phagocytosis of L. pneumophila by mammalian phagocytes.
已从嗜肺军团菌的四个血清群中分离出一种高分子量表面成分（F-1 组分）。通过微量凝集试验发现，针对活生物体产生的抗体对同源血清群具有特异性。抗血清与同源而非异源的 F-1 组分吸收后，其凝集活性显著降低。此外，还发现嗜肺军团菌在无抗血清的情况下不会被大鼠肺泡巨噬细胞或小鼠腹腔巨噬细胞吞噬，而同源抗血清能有效调理这些生物体。血清群特异性抗血清的调理活性通过用同源而非异源的 F-1 组分吸收抗血清而消除。这些数据表明，嗜肺军团菌的血清群特异性抗原存在于 F-1 组分中，并且 F-1 组分的抗体是哺乳动物吞噬细胞吞噬嗜肺军团菌所必需的。
