Lieberman M M, McKissock D C, Wright G L
Infect Immun. 1979 Feb;23(2):509-21. doi: 10.1128/iai.23.2.509-521.1979.
Passive protection of mice against Pseudomonas aeruginosa using specific antisera and immunoglobulin fractions induced by immunizing rabbits with a ribosomal vaccine is reported. The results demonstrated that protection by the ribosomal vaccine against challenge with live organisms can be serum mediated. Previous work has shown that the vaccine can be separated into two components on the basis of molecular weight and that both higher (peak A)- and lower (peak B)-molecular-weight fractions were capable of inducing active immunity in mice. The present report indicates that both fractions are also capable of eliciting the production of mouse-protective antibody in rabbits. Agar gel diffusion with antisera to peaks A and B or unfractionated vaccine indicated a common antigenic component among them in addition to an extra antigen in unfractionated vaccine not present in peak B. Passive hemagglutination with antisera to peaks A and B demonstrated high-titer agglutinating antibody only with antiserum to peak A when a method of erythrocyte sensitization for lipopolysaccharide antigens was used. Also, passive hemagglutination was greatly inhibited by small amounts of lipopolysaccharide prepared from the same organism from which the vaccine was made. Both antisera to peaks A and B fixed complement with either A or B antigens. Antisera to peaks A and B, when reacted with peak B antigen, had about the same complement fixation titer (as determined by a quantitative complement fixation test). However, when peak A antigen was used, antiserum to peak A had about twice the complement fixation titer that antiserum to peak B had. These results are consistent with previous observations which suggest that the ribosomal vaccine contains lipopolysaccharide in addition to an unidentified immunogenic principle associated with ribosomes. Furthermore, this immunogen was present in both peaks A and B, but detectable amounts of lipopolysaccharide were present only in peak A. The relative importance of the immunoglobulin G (IgG) and IgM classes of antibodies was also compared. The results indicated that both IgG and IgM isolated from immune rabbit serum are protective in mice. Only IgG precipitated with the vaccine in agar gel diffusion, but both IgG and IgM were active in passive hemagglutination and in complement fixation. The passive hemagglutination titer of the IgM was higher than that of the IgG, but the complement fixation titer of the IgG was higher than that of the IgM. The mouse-protective capability of the IgG and IgM was about the same.
据报道，用核糖体疫苗免疫兔子诱导产生的特异性抗血清和免疫球蛋白组分可对小鼠起到被动保护作用，使其免受铜绿假单胞菌感染。结果表明，核糖体疫苗对活生物体攻击的保护作用可通过血清介导。先前的研究表明，该疫苗可根据分子量分为两个组分，高分子量组分（峰A）和低分子量组分（峰B）都能够在小鼠中诱导主动免疫。本报告指出，这两个组分也能够在兔子中引发产生对小鼠有保护作用的抗体。用针对峰A和峰B的抗血清或未分级疫苗进行琼脂凝胶扩散试验表明，除了峰B中不存在的未分级疫苗中的额外抗原外，它们之间还有一个共同的抗原成分。当使用针对脂多糖抗原的红细胞致敏方法时，用针对峰A和峰B的抗血清进行被动血凝试验，结果显示只有针对峰A的抗血清产生高滴度凝集抗体。此外，由制备疫苗所用的同一生物体制备的少量脂多糖可极大地抑制被动血凝反应。针对峰A和峰B的抗血清均可与A或B抗原结合补体。针对峰A和峰B的抗血清与峰B抗原反应时，补体结合滴度大致相同（通过定量补体结合试验测定）。然而，当使用峰A抗原时，针对峰A的抗血清的补体结合滴度约为针对峰B的抗血清的两倍。这些结果与先前的观察结果一致，表明核糖体疫苗除了含有与核糖体相关的未鉴定免疫原性成分外，还含有脂多糖。此外，这种免疫原在峰A和峰B中均存在，但仅在峰A中存在可检测量的脂多糖。研究人员还比较了免疫球蛋白G（IgG）和IgM类抗体的相对重要性。结果表明，从免疫兔血清中分离出的IgG和IgM在小鼠中均具有保护作用。在琼脂凝胶扩散试验中，只有IgG与疫苗沉淀，但IgG和IgM在被动血凝反应和补体结合试验中均有活性。IgM的被动血凝滴度高于IgG，但IgG的补体结合滴度高于IgM。IgG和IgM对小鼠的保护能力大致相同。
