Zhang L N, Song Y T, Jiang L, Zhu R X, Li L
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
East China University of Political Science and Law, Shanghai 200063, China.
Fa Yi Xue Za Zhi. 2019 Feb;35(1):23-29. doi: 10.12116/j.issn.1004-5619.2019.01.005. Epub 2019 Feb 25.
To analyze the polymorphism of 71 SNP loci on Y chromosome in Han population of Northwest China, to assess its forensic application value, and to screen out Y-SNP loci for forensic examination of Han population in East, South, and Northwest China based on the integration of previous research results.
Multiplex polymerase chain reaction （PCR） and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） were performed on 71 Y-SNP loci of 202 unrelated Han male individuals in Northwest China. Gene diversity （GD） and haplotype diversity （HD） values were calculated, and then Y-SNP loci of Han population in East, South, and Northwest China were screened with the combination of data from previous research.
Among the detected 71 loci, 67 loci were polymorphic in the Northwest Han male population, with GD values 0.010 0-0.502 2. There were 22 and 25 loci with a moderate （0.2≤GD<0.3） and high （GD≥0.3） amount of genetic information, respectively. There were 26 loci for the Han communities in Northwest, South, and East China.
Y-SNP loci are potential in paternity testing and individual identification, as well as the judgement of population distribution and migration.
分析中国西北汉族人群Y染色体上71个单核苷酸多态性（SNP）位点的多态性，评估其法医学应用价值，并结合既往研究结果筛选出适用于中国东部、南部及西北汉族人群法医学检验的Y-SNP位点。
对中国西北202名无亲缘关系的汉族男性个体的71个Y-SNP位点进行多重聚合酶链反应（PCR）及基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）检测。计算基因多样性（GD）和单倍型多样性（HD）值，然后结合既往研究数据筛选中国东部、南部及西北汉族人群的Y-SNP位点。
在检测的71个位点中，67个位点在中国西北汉族男性人群中具有多态性，GD值为0.010 0-0.502 2。分别有22个和25个位点具有中等（0.2≤GD<0.3）和高（GD≥0.3）量的遗传信息。在中国西北、南部及东部汉族群体中有26个位点。
Y-SNP位点在亲子鉴定、个体识别以及人群分布和迁移判断方面具有潜在应用价值。
