Craig S S, Brink N G
School of Biological Sciences, Flinders University of South Australia, Australia.
Biol Cell. 1996;87(1-2):45-54.
Here we describe a new mutant, dosach (dos), in Drosophila melanogaster. In the mutant, centrosomes divide and initiate spindle formation similar to that seen in wild-type embryos. Nevertheless, mutant embryos form cleavage spindles that lack visible asters and display abnormal morphology, including mono- and tri-polar spindles, spindle chains and incorrect alignment. Irregular nuclear migration is also observed in mutant embryos, and this may suggest that astral microtubules are important for spindle spacing during cleavage and also in maintaining the integrity of the mitotic apparatus. Confocal microscopy has been used to correlate organization of microtubules, centrosomal proteins and chromosomes in wild-type and dosach (dos) embryos.
在此，我们描述了果蝇中的一种新突变体——多沙克（dosach，dos）。在该突变体中，中心体分裂并启动纺锤体形成，这与野生型胚胎中的情况相似。然而，突变体胚胎形成的分裂纺锤体缺乏可见的星体，并且呈现出异常形态，包括单极和三极纺锤体、纺锤体链以及排列不正确。在突变体胚胎中还观察到不规则的核迁移，这可能表明星体微管对于分裂过程中纺锤体的间距以及维持有丝分裂装置的完整性很重要。共聚焦显微镜已被用于关联野生型和多沙克（dosach，dos）胚胎中微管、中心体蛋白和染色体的组织情况。
