Epstein R S, Lopez D M, Sigel M M, Ross G D
J Immunol. 1978 Oct;121(4):1524-7.
In the course of mammary tumor development, a population of nylon nonadherent cells with CR appears in the spleens of tumor-bearing mice although none are ever detected in normal mice. These cells apparently arise in response to immunologic stimulation. In a series of studies we have further characterized subsets of T cells (CR+ and CR-) with regard to their responses to mitogens in the lymphocyte transformation assay. Nylon column nonadherent cells from the spleens of tumor-bearing mice were rosetted in a complement receptor assay using EAC rosetting, and CR+ cells were separated from CR- by centrifugation in a discontinuous Ficoll gradient. CR+ T cells responded strongly to PHA and Con A and in addition responded to LPS, an activity not usually associated with conventional T cells. In contrast, CR- T cells from tumor-burdened mice responded to PHA but failed to respond to Con A or LPS.
在乳腺肿瘤发展过程中，荷瘤小鼠脾脏中会出现一群带有补体受体（CR）的尼龙非黏附细胞，而正常小鼠中从未检测到此类细胞。这些细胞显然是对免疫刺激的反应而产生的。在一系列研究中，我们进一步根据T细胞亚群（CR⁺和CR⁻）在淋巴细胞转化试验中对丝裂原的反应特性进行了表征。使用EAC玫瑰花结试验，对荷瘤小鼠脾脏的尼龙柱非黏附细胞进行补体受体检测，通过在不连续的Ficoll梯度中离心，将CR⁺细胞与CR⁻细胞分离。CR⁺ T细胞对PHA和Con A反应强烈，此外还对LPS有反应，而LPS通常与传统T细胞无关。相比之下，荷瘤小鼠的CR⁻ T细胞对PHA有反应，但对Con A或LPS无反应。
