Department of Analytical Chemistry, Nutrition and Food Sciences, Institute for Food Analysis and Research-IIAA , Universidade de Santiago de Compostela , 15782 Santiago de Compostela , Spain.
Anal Chem. 2019 Apr 16;91(8):5176-5183. doi: 10.1021/acs.analchem.8b05792. Epub 2019 Mar 29.
The presence of persistent and mobile organic contaminants (PMOC) in aquatic environments has become a matter of concern due to their ability of breaking through natural and anthropogenic barriers, even reaching drinking water. The presence of many of these compounds in surface and drinking water has been reported in screening studies, but there is still a lack of analytical methods capable of quantifying them. Herein, we propose a method combining mixed-mode-solid-phase extraction (MM-SPE) as preconcentration technique and mixed-mode liquid chromatography (MMLC) coupled to tandem mass spectrometry as a determination technique for the quantitative determination of 23 target PMOCs in surface and drinking water samples. When compared to reversed-phase liquid chromatography, the MMLC protocol has proven to be superior in both retentive capabilities and peak shape for ionic compounds, while performing also well for neutrals. The overall method performance was satisfactory with limits of quantification under 50 ng L for most of analytes in both surface and drinking water. The relative standard deviation was lower than 20%, and the average recovery was 78 and 80% in surface and drinking water, respectively. The method was applied to 15 water samples collected in Spain, where 17 out of the 23 target PMOCs were quantified in at least one sample. Among them, 6 chemicals (e.g., benzyltrimethylammonium) are reported and/or quantified here for the first time.
由于持久性和移动有机污染物 (PMOC) 能够突破自然和人为屏障，甚至到达饮用水，因此它们在水生环境中的存在已成为一个令人关注的问题。在筛选研究中已经报道了许多这些化合物在地表水和饮用水中的存在，但仍缺乏能够对其进行定量的分析方法。在此，我们提出了一种将混合模式固相萃取 (MM-SPE) 作为预浓缩技术与混合模式液相色谱 (MMLC) 串联质谱结合的方法，用于定量测定地表水和饮用水样品中的 23 种目标 PMOC。与反相液相色谱相比，MMLC 方案在保留能力和离子化合物的峰形方面表现出色，同时对中性化合物也表现良好。该方法在地表水和饮用水中的大多数分析物的定量限均低于 50 ng L，整体方法性能令人满意。相对标准偏差低于 20%，地表水和饮用水的平均回收率分别为 78%和 80%。该方法应用于在西班牙采集的 15 个水样中，至少在一个样品中定量了 23 种目标 PMOC 中的 17 种。其中，有 6 种化学物质（例如苄基三甲基铵）是首次报道和/或定量的。
