Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco-UFRPE, Av. Dom Manoel de Medeiros, s/n, 52171-900 Recife, PE, Brazil.
Bioresour Technol. 2019 Jun;282:464-474. doi: 10.1016/j.biortech.2019.03.053. Epub 2019 Mar 12.
A new isolated P. citreonigrum URM 4459 was selected to produce fructooligosaccharides (FOS) in an efficient, economical and fast one-step fermentation. Optimal culture conditions were stablished by experimental design. Experiments run in bioreactor resulted in a high yield, content, productivity and purity of FOS (0.65 ± 0.06 g/g, 126.3 ± 0.1 g/L, 2.28 ± 0.08 g/L.h and 61 ± 0%). The FOS mixture was purified up to 92% (w/w) with an activated charcoal column. FOS produced were able to promote lactobacilli and bifidobacteria growth. Higher bacteria cell density was obtained for microbial-FOS mixtures than commercial Raftilose P95. Some strains grew even faster in the FOS mixture produced than in all other carbon sources. FOS were resistant to the simulated gastrointestinal conditions. A high amount of a reducing trisaccharide was identified in the FOS produced mixture, possibly neokestose, which may explain the great prebiotic potential of the FOS.
一株新的分离出的青霉菌 P. citreonigrum URM 4459 被选中用于在高效、经济和快速的一步发酵中生产低聚果糖 (FOS)。通过实验设计确定了最佳的培养条件。在生物反应器中的实验产生了高产量、含量、生产率和 FOS 纯度 (0.65 ± 0.06 g/g、126.3 ± 0.1 g/L、2.28 ± 0.08 g/L.h 和 61 ± 0%)。FOS 混合物用活性炭柱纯化至 92%（w/w）。所产生的 FOS 能够促进乳酸菌和双歧杆菌的生长。与商业 Raftilose P95 相比，微生物-FOS 混合物获得了更高的细菌细胞密度。一些菌株在 FOS 混合物中的生长速度甚至比其他所有碳源都快。FOS 能够抵抗模拟的胃肠道条件。在产生的 FOS 混合物中鉴定出了大量的还原三糖，可能是新海藻糖，这可能解释了 FOS 的巨大益生元潜力。
