优化稀硫酸、氨水和蒸汽爆破预处理工艺，以提高可发酵性干酒糟及其可溶物作为潜在发酵原料的性能。

Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock.

机构信息

Department of Agricultural and Biological Engineering, Pennsylvania State University, University Park, PA 16802, USA.

Department of Food Engineering, Middle East Technical University, 06800 Ankara, Turkey.

出版信息

Bioresour Technol. 2019 Jun;282:475-481. doi: 10.1016/j.biortech.2019.03.009. Epub 2019 Mar 5.

DOI:10.1016/j.biortech.2019.03.009

PMID:30897485

Abstract

Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreatment at 121 °C is the most effective way of obtaining simple fermentable sugars (0.382 g/g DDGS). The levels of furfural and HMF was also 5.2 mg/g DDGS) and 1.6 mg/g DDGS, respectively, in the dilute sulfuric acid pretreated DDGS. On the other hand, maximum sugar yield for ammonia pretreatment was 0.129 g/g DDGS and 0.055 g/g DDGS for the steam pretreatment, while no significant amounts of furfural and HMF were observed for these two pretreatment methods.

摘要

酒糟干燥物（DDGS）是通过干磨发酵从富含淀粉的谷物生产生物乙醇的副产品。在本研究中，优化了稀酸水解、氨水和蒸汽爆破等预处理方法。采用中心复合响应面法（RSM）设计对稀酸预处理、氨水处理进行了优化。评估了蒸汽爆破试验。结果表明，在 121°C 下进行稀酸预处理是获得简单可发酵糖（0.382g/g DDGS）的最有效方法。在稀酸预处理的 DDGS 中，糠醛和 5-羟甲基糠醛的含量分别为 5.2mg/g DDGS 和 1.6mg/g DDGS。另一方面，氨预处理的最大糖收率为 0.129g/g DDGS，蒸汽预处理的糖收率为 0.055g/g DDGS，而这两种预处理方法均未观察到糠醛和 5-羟甲基糠醛的显著含量。