生长激素释放因子在清醒大鼠中5-羟色胺能机制诱导的生长激素分泌中的作用。

Involvement of growth hormone (GH)-releasing factor in GH secretion induced by serotoninergic mechanisms in conscious rats.

作者信息

Murakami Y, Kato Y, Kabayama Y, Tojo K, Inoue T, Imura H

出版信息

Endocrinology. 1986 Sep;119(3):1089-92. doi: 10.1210/endo-119-3-1089.

Abstract

A possible role of hypothalamic GH-releasing factor (GRF) in GH secretion induced by serotoninergic stimuli was investigated in conscious freely moving rats with a passive immunization technique. Either iv injection of 5-hydroxy-L-tryptophan (1 mg/100 g BW) or intracerebroventricular injection of serotonin (10 micrograms/rat) caused an increase in plasma GH in rats treated with normal rabbit serum (0.5 ml/rat, iv) 30 min previously. Injection of rabbit antiserum specific for rat GRF (0.5 ml/rat, iv, 30 min previously) blunted the plasma GH increase induced by 5-hydroxy-L-tryptophan or serotonin in these animals. These results suggest that GH secretion induced by serotoninergic mechanisms is mediated, at least in part, by hypothalamic GRF in the rat.

摘要

采用被动免疫技术，在清醒自由活动的大鼠中研究了下丘脑生长激素释放因子（GRF）在5-羟色胺能刺激诱导的生长激素分泌中的可能作用。静脉注射5-羟-L-色氨酸（1mg/100g体重）或脑室内注射5-羟色胺（10μg/只大鼠）可使30分钟前经静脉注射正常兔血清（0.5ml/只大鼠）处理的大鼠血浆生长激素水平升高。注射针对大鼠GRF的兔抗血清（0.5ml/只大鼠，静脉注射，30分钟前）可减弱这些动物中由5-羟-L-色氨酸或5-羟色胺诱导的血浆生长激素升高。这些结果表明，5-羟色胺能机制诱导的生长激素分泌至少部分是由大鼠下丘脑GRF介导的。