Salvador Lorena, Guijarro Michelle, Rubio Daniela, Aucatoma Bolívar, Guillén Tanya, Vargas Jentzsch Paul, Ciobotă Valerian, Stolker Linda, Ulic Sonia, Vásquez Luis, Garrido Patricia, Bravo Juan, Ramos Guerrero Luis
Centro de Investigación de Alimentos, CIAL, Universidad UTE, EC170527 Quito, Ecuador.
Centro de Investigación de la Caña de Azúcar, CINCAE, El Triunfo 091601, Ecuador.
Foods. 2019 Mar 20;8(3):105. doi: 10.3390/foods8030105.
Honey is one of the oldest sweetening foods and has economic importance, making this product attractive to adulteration with cheap sugars. This can cause a critical problem in the honey industry and a possible health risk. The present work has the aim of evaluating the authenticity of honey commercialized in two different provinces of Ecuador (Pichincha and Loja) by performing physicochemical and spectroscopic analyses. For this study 25 samples were collected from different places and markets and characterized by water, sucrose, reducing sugars and electric conductivity measurement. Also, their Raman and Infrared (IR) spectra were recorded and analysed using a Principal Component Analysis (PCA) in order to verify the quality of the honeys. In addition, a screening of several pesticides was performed in order to verify possible chemical threats to human health and honey bees. It was found that 8 samples have a deviation from the Standard established parameters. Two of them have a high difference in the content of sucrose and reducing sugars, which are located deviated from all the other samples in the PCA of the applied vibrational spectroscopy (IR/Raman), shaping two clear clusters. The results show that Raman and IR spectroscopy is appropriate techniques for the quality control of honey and correlates well with the physicochemical analyses.
蜂蜜是最古老的甜味食品之一，具有经济重要性，这使得该产品容易受到用廉价糖类掺假的影响。这可能会给蜂蜜行业带来严重问题，并带来潜在的健康风险。本研究旨在通过进行物理化学和光谱分析，评估在厄瓜多尔两个不同省份（皮钦查省和洛哈省）商业化销售的蜂蜜的真伪。在本研究中，从不同地点和市场收集了25个样本，并通过测量水分、蔗糖、还原糖和电导率进行表征。此外，记录并使用主成分分析（PCA）对它们的拉曼光谱和红外（IR）光谱进行分析，以验证蜂蜜的质量。另外，还对几种农药进行了筛查，以验证对人类健康和蜜蜂可能存在的化学威胁。结果发现，有8个样本偏离了既定标准参数。其中两个样本的蔗糖和还原糖含量差异很大，在应用振动光谱（红外/拉曼）的主成分分析中，它们与所有其他样本偏离，形成了两个明显的聚类。结果表明，拉曼光谱和红外光谱是用于蜂蜜质量控制的合适技术，并且与物理化学分析相关性良好。
