从人凝血因子VIII/血管性血友病因子中分离出的一种新型N-乙酰乳糖胺型四天线聚糖的一级结构。
Primary structure of a new tetraantennary glycan of the N-acetyllactosaminic type isolated from human factor VIII/von Willebrand factor.
作者信息
Samor B, Michalski J C, Debray H, Mazurier C, Goudemand M, Van Halbeek H, Vliegenthart J F, Montreuil J
出版信息
Eur J Biochem. 1986 Jul 15;158(2):295-8. doi: 10.1111/j.1432-1033.1986.tb09750.x.
N-Glycosidically linked glycopeptides released by mild alkaline treatment of human factor VIII/von Willebrand factor (FVIII/vWF) were fractionated by serial affinity chromatography on columns of Sepharose linked to concanavalin A (ConA) and Lens culinaris agglutinin (LCA). The fraction which is not retained on ConA-Sepharose, but eluted from LCA-Sepharose contains a pure minor glycopeptide which was structurally analysed. Based on the results of methylation analysis and 500-MHz 1H-NMR spectroscopy, the following structure is proposed: (Formula: see text).
通过对人因子VIII/血管性血友病因子（FVIII/vWF）进行温和碱性处理释放出的N-糖苷键连接的糖肽，在与伴刀豆球蛋白A（ConA）和扁豆凝集素（LCA）偶联的琼脂糖柱上进行连续亲和层析分离。未保留在ConA-琼脂糖上但从LCA-琼脂糖上洗脱下来的部分含有一种纯的次要糖肽，并对其进行了结构分析。根据甲基化分析和500兆赫兹1H-NMR光谱的结果，提出了以下结构：（分子式：见原文）。
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