人血管性血友病因子主要O-糖苷键连接碳水化合物单元的一级结构。
Primary structure of the major O-glycosidically linked carbohydrate unit of human von Willebrand factor.
作者信息
Samor B, Michalski J C, Mazurier C, Goudemand M, De Waard P, Vliegenthart J F, Strecker G, Montreuil J
机构信息
Centre Régional de Transfusion Sanguine, laboratoire d'Hémostase, Lille, France.
出版信息
Glycoconj J. 1989;6(3):263-70. doi: 10.1007/BF01047846.
A reduced tetrasaccharide chain was obtained from human von Willebrand factor (vWF) by mild alkaline borohydride treatment. The purification of this O-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin A and Lens culinaris agglutinin and finally gel filtration. Its structure was determined by a combination of methylation studies and 500 MHz 1H-NMR spectroscopy to be: NeuAc(alpha 2-3)Gal(beta 1-3)[NeuAc(alpha 2-6)]GalNAc-ol.
通过温和的碱性硼氢化物处理从人血管性血友病因子（vWF）中获得了一条缩短的四糖链。通过在固定化伴刀豆球蛋白A和扁豆凝集素上进行连续亲和层析，最后进行凝胶过滤，实现了这种O-糖苷键连接的寡糖的纯化。通过甲基化研究和500兆赫1H-NMR光谱相结合确定其结构为：NeuAc（α2-3）Gal（β1-3）[NeuAc（α2-6）]GalNAc-ol。
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