Food Science and Nutrition Dept., Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Espinardo, Spain; Special Food and Nutrition Dept., Food Technology Research Institute (FTRI), Agricultural Research Center (ARC), 12619 Giza, Egypt.
Food Science and Nutrition Dept., Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Espinardo, Spain.
Food Res Int. 2019 Apr;118:101-107. doi: 10.1016/j.foodres.2017.12.029. Epub 2017 Dec 14.
This study aimed to evaluate the potential anti-inflammatory role of the most produced form of lactoferrin expressed in various expression systems (Fe-saturated recombinant human Lf, rhLf) and its hydrolysate in concentrations resembles that found in mature human milk. Co-culture model consisted of CaCo-2 and RAW 246.7 cell lines was used to evaluate the potential anti-inflammatory activity of rhLf and its hydrolysate. During this experiment, CaCo-2 monolayer permeability and integrity was assayed through the measurement of transepithelial electrical resistance (TEER values). Also, the production of reactive oxygen species (ROS), nitric oxide (NO) and different cytokines (IL-8, IL-1β, IL-6, IL-10, IL-12p70, and TNF-α) were measured. The treatment with rhLf and its hydrolysate protected the monolayer integrity against LPS effect and reduced IL-8 and ROS production. This effect was dependent on the dose and 2mgmL of rhLf hydrolysate was more effective. The addition of rhLf and its hydrolysate to infant formula is a prominent step towards improving both infant formula functionality and newborn health. Thus, these functional ingredients could be incorporated in infant foods. In this context, ongoing researches are conducted to clarify this effect whether by using synthetic peptides or by using LPS-sepsis animal.
本研究旨在评估在各种表达系统中表达的最常见形式的乳铁蛋白（Fe 饱和重组人乳铁蛋白，rhLf）及其水解产物在类似于成熟人乳中发现的浓度下的潜在抗炎作用。使用共培养模型 CaCo-2 和 RAW 246.7 细胞系来评估 rhLf 及其水解产物的潜在抗炎活性。在这个实验中，通过测量跨上皮电阻（TEER 值）来检测 CaCo-2 单层通透性和完整性。同时，还测量了活性氧（ROS）、一氧化氮（NO）和不同细胞因子（IL-8、IL-1β、IL-6、IL-10、IL-12p70 和 TNF-α）的产生。rhLf 和其水解产物的处理保护了单层完整性免受 LPS 的影响，并减少了 IL-8 和 ROS 的产生。这种作用依赖于剂量，2mgmL 的 rhLf 水解产物更有效。在婴儿配方奶粉中添加 rhLf 和其水解产物是改善婴儿配方奶粉功能和新生儿健康的重要步骤。因此，这些功能性成分可以添加到婴儿食品中。在这种情况下，正在进行研究以阐明这种作用，无论是使用合成肽还是使用 LPS 败血症动物。
