NELL-1 亚型对骨髓基质细胞的年龄依赖性影响。
Age dependent effects of NELL-1 isoforms on bone marrow stromal cells.
作者信息
Meyers Carolyn A, Sun Zhibo, Chang Leslie, Ding Catherine, Lu Amy, Ting Kang, Pang Shen, James Aaron W
机构信息
Department of Pathology, Johns Hopkins University, 21205, USA.
Division of Growth and Development, School of Dentistry, University of California, Los Angeles, USA.
出版信息
J Orthop. 2019 Feb 28;16(2):175-178. doi: 10.1016/j.jor.2019.02.006. eCollection 2019 Mar-Apr.
NELL-1 is an osteogenic protein first discovered to control ossification of the cranium. NELL-1 exists in at least two isoforms. The full-length NELL-1 contains 810 amino acid (aa) (NELL-1810), the N-terminal-truncated NELL-1 isoform contains 570 aa (NELL-1570). The differences in cellular effects between NELL-1 isoforms are not well understood. Methods: Here, BMSC were derived from adult or aged mice, followed by overexpression of NELL-1810 or NELL-1570. Cell morphology, proliferation, and gene expression were examined. Results/Conclusions: Overall, the proliferative effect of NELL-1570 was age dependent, showing prominent induction in adult but not aged mice.
NELL-1是一种首先被发现可控制颅骨骨化的成骨蛋白。NELL-1至少以两种异构体形式存在。全长NELL-1含有810个氨基酸（aa）（NELL-1810），N端截短的NELL-1异构体含有570个aa（NELL-1570）。NELL-1异构体之间细胞效应的差异尚不清楚。方法：在此，从成年或老年小鼠中分离出骨髓间充质干细胞（BMSC），然后过表达NELL-1810或NELL-1570。检测细胞形态、增殖和基因表达。结果/结论：总体而言，NELL-1570的增殖作用具有年龄依赖性，在成年小鼠中表现出显著诱导作用，而在老年小鼠中则不然。
Zotero 插件