Leo Vincent Vineeth, Passari Ajit Kumar, Muniraj Iniya Kumar, Uthandi Sivakumar, Hashem Abeer, Abd Allah Elsayed Fathi, Alqarawi Abdulaziz A, Singh Bhim Pratap
Department of Biotechnology, Aizawl, Mizoram University, Mizoram 796004, India.
Department of Biotechnology, J.J College for Arts and Science, Pudukkottai, Tamil Nadu, India.
Saudi J Biol Sci. 2019 Mar;26(3):464-468. doi: 10.1016/j.sjbs.2018.10.006. Epub 2018 Oct 6.
Laccases (EC 1.10.3.2) are a class of multi-copper oxidases that have industrial value. In the present study, forty-five isolates of wild mushrooms were screened for laccase production. Eight of the isolates exhibited exploitable levels of substrate oxidation capacity. Isolate BPSM10 exhibited the highest laccase activity of 103.50 U/ml. Internal Transcribed Spacer (ITS) rRNA gene sequencing was used to identify BPSM10 as . The response of BPSM10 to two nutritional media supplemented with various inducers was characterized and the results indicated that Malt Extract Broth (MEB) supplemented with Xylidine increased laccase production by 2.8× (349.5 U/ml) relative to the control (122 U/ml), while Potato Dextrose Broth (PDB) supplemented with xylidine increased laccase production by 1.9× (286 U/ml). BPSM10 has the ability to decolorize seven synthetic dyes in a liquid medium. Maximum decolorization was observed of malachite green (MG); exhibiting 68.6% decolorization at 100 mg/L. Fourier-transform infrared spectroscopy (FTIR) was employed to confirm the decolorization capacity. The present study indicates that BPSM10 has the potential to be used as a potent alternative biosorbent for the removal of synthetic dyes from aqueous solutions, especially in the detoxification of polluted water.
漆酶（EC 1.10.3.2）是一类具有工业价值的多铜氧化酶。在本研究中，对45株野生蘑菇分离株进行了漆酶产生情况的筛选。其中8株分离株表现出可利用水平的底物氧化能力。分离株BPSM10表现出最高的漆酶活性，为103.50 U/ml。利用内转录间隔区（ITS）rRNA基因测序将BPSM10鉴定为 。对BPSM10在两种添加了各种诱导剂的营养培养基中的反应进行了表征，结果表明，添加二甲苯胺的麦芽浸出液肉汤（MEB）使漆酶产量相对于对照（122 U/ml）提高了2.8倍（349.5 U/ml），而添加二甲苯胺的马铃薯葡萄糖肉汤（PDB）使漆酶产量提高了1.9倍（286 U/ml）。BPSM10能够在液体培养基中使7种合成染料脱色。孔雀石绿（MG）的脱色效果最佳；在100 mg/L时脱色率达68.6%。采用傅里叶变换红外光谱（FTIR）来确认脱色能力。本研究表明，BPSM10有潜力作为一种有效的替代生物吸附剂用于从水溶液中去除合成染料，特别是在污染水的解毒方面。
