Concetti A, Raffioni S, Miceli C, Barra D, Luporini P
J Biol Chem. 1986 Aug 15;261(23):10582-6.
Mating type-specific mating pheromones autonomously released into the environment control cell-cell union in conjugation of the marine ciliate Euplotes raikovi. The mating pheromone, termed euplomone r-1, of cells of the homozygous mating type determined by the mat-1/mat-1 genotype was purified by means of a three-stage purification procedure which provides a yield of 79%. Starting from 10 liters of supernatant, 3.3 mg of euplomone r-1 were regularly recovered. Euplomone r-1 was identified with a protein showing a molecular weight of 12,000, an isoelectric point of 3.7, and unusually high contents of cysteine (15%) and tyrosine (5.8%). It appeared active at a concentration of 3.4 +/- 0.5 X 10(-12) M. Carbohydrate and a small colored compound, not yet identified, occurred in association with partially purified euplomone r-1 samples, whereas they were not detected in samples of the final product of purification. An acidic shift was apparently capable of destroying the carbohydrate/euplomone r-1 association, suggesting that it does not involve covalent bonds.
在海洋纤毛虫雷氏游仆虫的接合过程中，特定交配型的交配信息素自主释放到环境中，控制细胞间的结合。通过三步纯化程序纯化了由mat-1/mat-1基因型决定的纯合交配型细胞的交配信息素，称为优普洛蒙r-1，产率为79%。从10升上清液开始，通常可回收3.3毫克优普洛蒙r-1。优普洛蒙r-1被鉴定为一种蛋白质，其分子量为12000，等电点为3.7，半胱氨酸（15%）和酪氨酸（5.8%）含量异常高。它在浓度为3.4±0.5×10⁻¹² M时表现出活性。碳水化合物和一种尚未鉴定的小的有色化合物与部分纯化的优普洛蒙r-1样品相关联，而在纯化终产物样品中未检测到它们。酸性变化显然能够破坏碳水化合物/优普洛蒙r-1的关联，这表明它不涉及共价键。
