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纤毛虫游仆虫交配诱导因子的分离

Isolation of the mating-inducing factor of the ciliate Euplotes.

作者信息

Miceli C, Concetti A, Luporini P

出版信息

Exp Cell Res. 1983 Dec;149(2):593-8. doi: 10.1016/0014-4827(83)90371-3.

DOI:10.1016/0014-4827(83)90371-3
PMID:6416879
Abstract

Numerous strains of different mating types of the marine ciliate Euplotes raikovi have been found to be autonomous excreters into the surrounding medium of specific mating-inducing factors (gamones) (Luporini, P et al., J exp zool 226 (1983) 1 [9]). The gamone from the mating type represented by strain 13 has been isolated and identified as a glycoprotein with a molecular weight (MW) of about 12 kD and a pI of 4. It has been termed euplomone r 13. At a concentration of 3 X 10(-12) M, euplomone r 13 specifically induces cells of a complementary mating type to unite in conjugation within 2 h.

摘要

已发现海洋纤毛虫雷氏游仆虫(Euplotes raikovi)不同交配型的许多菌株能自主向周围介质分泌特定的交配诱导因子(配子素)(卢波里尼,P等人,《实验动物学杂志》226卷(1983年)第1页[9])。来自13号菌株所代表的交配型的配子素已被分离出来,并被鉴定为一种糖蛋白,分子量约为12千道尔顿,等电点为4。它被命名为游仆配子素r 13。在浓度为3×10⁻¹² M时,游仆配子素r 13能在2小时内特异性诱导互补交配型的细胞结合进行接合。

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引用本文的文献

1
The ciliate Euplotes raikovi heterozygous at the mat genetic locus coreleases two individual species of mating pheromone: Genetic and biochemical evidence.纤毛虫雷科夫异质体在 mat 遗传基因座上核心释放两种单独的交配信息素物种:遗传和生化证据。
Proc Natl Acad Sci U S A. 1986 May;83(9):2889-93. doi: 10.1073/pnas.83.9.2889.
2
Isolation and structural characterization of cDNA clones encoding the mating pheromone Er-1 secreted by the ciliate Euplotes raikovi.编码纤毛虫雷氏游仆虫分泌的交配信息素Er-1的cDNA克隆的分离与结构表征。
Proc Natl Acad Sci U S A. 1989 May;86(9):3016-20. doi: 10.1073/pnas.86.9.3016.
3
Identification and initial characterization of an autocrine pheromone receptor in the protozoan ciliate Euplotes raikovi.
原生动物纤毛虫雷氏游仆虫中自分泌信息素受体的鉴定及初步表征
J Cell Biol. 1990 Aug;111(2):607-14. doi: 10.1083/jcb.111.2.607.
4
Primary structure of Euplotes raikovi pheromones: comparison of five sequences of pheromones from cells with variable mating interactions.雷氏游仆虫信息素的一级结构:具有可变交配相互作用的细胞中五种信息素序列的比较
Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2071-5. doi: 10.1073/pnas.89.6.2071.