Ambrosino D M, Barrus V A, DeLange G G, Siber G R
J Clin Invest. 1986 Aug;78(2):361-5. doi: 10.1172/JCI112585.
Km allotype antigens are serologic markers expressed on kappa light chains of human immunoglobulins. To determine whether th Km phenotype of an individual is related to his ability to make antibodies to polysaccharide antigens, we correlated the Km allotypes of 129 healthy caucasian adults with the concentrations of specific antibodies to three bacterial polysaccharide antigens after immunization. The 14 individuals expressing the Km(1) allotype had lower concentrations of IgG, IgM, and IgA antibodies by enzyme-linked immunosorbent assay and total antibody by radioimmunoassay to Haemophilus influenzae type b and Neisseria meningitidis group C capsular polysaccharides when compared with the 115 Km(1) negative individuals. The Km-associated differences in H. influenzae type b and N. meningitidis group C antibody concentrations were confined to kappa light chain-containing antibody (P = 0.029 and 0.003, respectively). Similarly, the Km(1) positives had slightly lower kappa chain-containing Ig than the Km(1) negatives (P = 0.079). We conclude that genes in or near the kappa light chain locus play a role in the regulation of kappa-containing antibody production to some bacterial polysaccharides and perhaps to other antigens.
Km同种异型抗原是在人类免疫球蛋白κ轻链上表达的血清学标志物。为了确定个体的Km表型是否与其产生针对多糖抗原的抗体的能力有关，我们将129名健康白种成年人的Km同种异型与免疫后针对三种细菌多糖抗原的特异性抗体浓度进行了关联分析。通过酶联免疫吸附测定法，表达Km(1)同种异型的14名个体针对b型流感嗜血杆菌和C群脑膜炎奈瑟菌荚膜多糖的IgG、IgM和IgA抗体浓度以及通过放射免疫测定法测得的总抗体浓度，均低于115名Km(1)阴性个体。b型流感嗜血杆菌和C群脑膜炎奈瑟菌抗体浓度与Km相关的差异仅限于含κ轻链的抗体（分别为P = 0.029和0.003）。同样，Km(1)阳性个体含κ链的Ig略低于Km(1)阴性个体（P = 0.079）。我们得出结论，κ轻链基因座内或附近的基因在调节针对某些细菌多糖以及可能针对其他抗原的含κ抗体产生中发挥作用。
